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Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(10), 1557; doi:10.3390/ijms17101557

Virus-Induced Gene Silencing Identifies an Important Role of the TaRSR1 Transcription Factor in Starch Synthesis in Bread Wheat

1
,
1,2
,
2,3
,
2,3
,
1,2
,
1,3
,
1,3,* and 1,2,3,*
1
The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China
2
The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China
3
The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Lam-Son Phan Tran and Jianhua Zhu
Received: 8 July 2016 / Revised: 28 August 2016 / Accepted: 7 September 2016 / Published: 23 September 2016
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [3529 KB, uploaded 23 September 2016]   |  

Abstract

The function of a wheat starch regulator 1 (TaRSR1) in regulating the synthesis of grain storage starch was determined using the barley stripe mosaic virus—virus induced gene-silencing (BSMV-VIGS) method in field experiments. Chlorotic stripes appeared on the wheat spikes infected with barley stripe mosaic virus-virus induced gene-silencing- wheat starch regulator 1 (BSMV-VIGS-TaRSR1) at 15 days after anthesis, at which time the transcription levels of the TaRSR1 gene significantly decreased. Quantitative real-time PCR was also used to measure the transcription levels of 26 starch synthesis-related enzyme genes in the grains of BSMV-VIGS-TaRSR1-silenced wheat plants at 20, 27, and 31 days after anthesis. The results showed that the transcription levels of some starch synthesis-related enzyme genes were markedly induced at different sampling time points: TaSSI, TaSSIV, TaBEIII, TaISA1, TaISA3, TaPHOL, and TaDPE1 genes were induced at each of the three sampling time points and TaAGPS1-b, TaAGPL1, TaAGPL2, TaSSIIb, TaSSIIc, TaSSIIIb, TaBEI, TaBEIIa, TaBEIIb, TaISA2, TaPHOH, and TaDPE2 genes were induced at one sampling time point. Moreover, both the grain starch contents, one thousand kernel weights, grain length and width of BSMV-VIGS-TaRSR1-infected wheat plants significantly increased. These results suggest that TaRSR1 acts as a negative regulator and plays an important role in starch synthesis in wheat grains by temporally regulating the expression of specific starch synthesis-related enzyme genes. View Full-Text
Keywords: TaRSR1; Triticum aestivum L.; BSMV-VIGS; starch synthesis regulation TaRSR1; Triticum aestivum L.; BSMV-VIGS; starch synthesis regulation
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    Description: Figure S1. cDNA sequences for three copies of f TaRSR1 gene. The fragment used to VIGS silencing has been underlined. Figure S2. Transcription levels of the TaRSR1 gene in the BSMV-TaRSR1-infected and BSMV-GFP-infected wheat plants. Figure S3. Transcription levels of the 26 starch synthesis enzyme genes in the grains of BSMV-TaRSR1-infected and BSMV-GFP-infected wheat plants. Figure S4. Schematic construction of the recombinant BSMV-TaRSR1 vector. Figure S5. BSMV-TaRSR1 and BSMV-GFP virus inoculation under field conditions. Table S1. Target genes for analyzing transcription levels and their primer sequences.

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Liu, G.; Wu, Y.; Xu, M.; Gao, T.; Wang, P.; Wang, L.; Guo, T.; Kang, G. Virus-Induced Gene Silencing Identifies an Important Role of the TaRSR1 Transcription Factor in Starch Synthesis in Bread Wheat. Int. J. Mol. Sci. 2016, 17, 1557.

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