Next Article in Journal / Special Issue
The Clinical Significance of Phosphorylated Heat Shock Protein 27 (HSPB1) in Pancreatic Cancer
Previous Article in Journal
Structure Prediction: New Insights into Decrypting Long Noncoding RNAs
Previous Article in Special Issue
The Functional Analysis of Histone Acetyltransferase MOF in Tumorigenesis
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(1), 69; doi:10.3390/ijms17010069

Unraveling Molecular Differences of Gastric Cancer by Label-Free Quantitative Proteomics Analysis

1
State Key Laboratory of Cancer Biology, Department of Pharmacogenomics, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China
2
The Cancer Research Center, School of Medicine, Shandong University, Jinan 250012, China
3
Departments of Surgery and Urology, Boston Veterans Affairs Healthcare System, Boston University School of Medicine, Boston, MA 02130, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 10 November 2015 / Revised: 16 December 2015 / Accepted: 25 December 2015 / Published: 21 January 2016
View Full-Text   |   Download PDF [4916 KB, uploaded 21 January 2016]   |  

Abstract

Gastric cancer (GC) has significant morbidity and mortality worldwide and especially in China. Its molecular pathogenesis has not been thoroughly elaborated. The acknowledged biomarkers for diagnosis, prognosis, recurrence monitoring and treatment are lacking. Proteins from matched pairs of human GC and adjacent tissues were analyzed by a coupled label-free Mass Spectrometry (MS) approach, followed by functional annotation with software analysis. Nano-LC-MS/MS, quantitative real-time polymerase chain reaction (qRT-PCR), western blot and immunohistochemistry were used to validate dysregulated proteins. One hundred forty-six dysregulated proteins with more than twofold expressions were quantified, 22 of which were first reported to be relevant with GC. Most of them were involved in cancers and gastrointestinal disease. The expression of a panel of four upregulated nucleic acid binding proteins, heterogeneous nuclear ribonucleoprotein hnRNPA2B1, hnRNPD, hnRNPL and Y-box binding protein 1 (YBX-1) were validated by Nano-LC-MS/MS, qRT-PCR, western blot and immunohistochemistry assays in ten GC patients’ tissues. They were located in the keynotes of a predicted interaction network and might play important roles in abnormal cell growth. The label-free quantitative proteomic approach provides a deeper understanding and novel insight into GC-related molecular changes and possible mechanisms. It also provides some potential biomarkers for clinical diagnosis. View Full-Text
Keywords: gastric cancer; LC-MS/MS; Label-free quantitative proteomics; heterogeneous nuclear ribonucleoprotein; Y-box binding protein 1 gastric cancer; LC-MS/MS; Label-free quantitative proteomics; heterogeneous nuclear ribonucleoprotein; Y-box binding protein 1
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Dai, P.; Wang, Q.; Wang, W.; Jing, R.; Wang, W.; Wang, F.; Azadzoi, K.M.; Yang, J.-H.; Yan, Z. Unraveling Molecular Differences of Gastric Cancer by Label-Free Quantitative Proteomics Analysis. Int. J. Mol. Sci. 2016, 17, 69.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top