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Int. J. Mol. Sci. 2016, 17(1), 57; doi:10.3390/ijms17010057

Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca2+, CaM and Depolarization of Plasma Membrane Potential

1
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
2
School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
3
College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095 China
4
School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Hatem Rouached
Received: 4 December 2015 / Revised: 20 December 2015 / Accepted: 22 December 2015 / Published: 5 January 2016
(This article belongs to the Special Issue Plant Molecular Biology)
View Full-Text   |   Download PDF [7297 KB, uploaded 5 January 2016]   |  

Abstract

Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca2+ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca2+ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca2+ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca2+ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca2+ and H+ efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca2+-CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants. View Full-Text
Keywords: Ca2+ efflux; Ca2+ fluorescence; CaM; fluoride; NPPB; tea plant Ca2+ efflux; Ca2+ fluorescence; CaM; fluoride; NPPB; tea plant
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, X.-C.; Gao, H.-J.; Yang, T.-Y.; Wu, H.-H.; Wang, Y.-M.; Zhang, Z.-Z.; Wan, X.-C. Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca2+, CaM and Depolarization of Plasma Membrane Potential. Int. J. Mol. Sci. 2016, 17, 57.

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