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Int. J. Mol. Sci. 2015, 16(9), 22319-22332; doi:10.3390/ijms160922319

Digital Microfluidics for Manipulation and Analysis of a Single Cell

Department of Mechanical Engineering, National Taiwan University, Taipei 10617, Taiwan
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Author to whom correspondence should be addressed.
Academic Editors: Fan-Gang Tseng and Tuhin Subhra Santra
Received: 31 May 2015 / Revised: 12 August 2015 / Accepted: 18 August 2015 / Published: 15 September 2015
(This article belongs to the Special Issue Single Cell Analysis in Biotechnology and Systems Biology)
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Abstract

The basic structural and functional unit of a living organism is a single cell. To understand the variability and to improve the biomedical requirement of a single cell, its analysis has become a key technique in biological and biomedical research. With a physical boundary of microchannels and microstructures, single cells are efficiently captured and analyzed, whereas electric forces sort and position single cells. Various microfluidic techniques have been exploited to manipulate single cells through hydrodynamic and electric forces. Digital microfluidics (DMF), the manipulation of individual droplets holding minute reagents and cells of interest by electric forces, has received more attention recently. Because of ease of fabrication, compactness and prospective automation, DMF has become a powerful approach for biological application. We review recent developments of various microfluidic chips for analysis of a single cell and for efficient genetic screening. In addition, perspectives to develop analysis of single cells based on DMF and emerging functionality with high throughput are discussed. View Full-Text
Keywords: microfluidic chips; digital microfluidics; single cell analysis; genetic screening; assisted reproductive technologies microfluidic chips; digital microfluidics; single cell analysis; genetic screening; assisted reproductive technologies
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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He, J.-L.; Chen, A.-T.; Lee, J.-H.; Fan, S.-K. Digital Microfluidics for Manipulation and Analysis of a Single Cell. Int. J. Mol. Sci. 2015, 16, 22319-22332.

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