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Int. J. Mol. Sci. 2015, 16(9), 21310-21329; doi:10.3390/ijms160921310

Gene Expression Variations of Red—White Skin Coloration in Common Carp (Cyprinus carpio)

1
CAFS Key Laboratory of Aquatic Genomics and Beijing Key Laboratory of Fishery Biotechnology, Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 10014, China
2
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
3
Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 16 July 2015 / Revised: 14 August 2015 / Accepted: 25 August 2015 / Published: 7 September 2015
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [1930 KB, uploaded 7 September 2015]   |  

Abstract

Teleosts have more types of chromatophores than other vertebrates and the genetic basis for pigmentation is highly conserved among vertebrates. Therefore, teleosts are important models to study the mechanism of pigmentation. Although functional genes and genetic variations of pigmentation have been studied, the mechanisms of different skin coloration remains poorly understood. The koi strain of common carp has various colors and patterns, making it a good model for studying the genetic basis of pigmentation. We performed RNA-sequencing for red skin and white skin and identified 62 differentially expressed genes (DEGs). Most of them were validated with RT-qPCR. The up-regulated DEGs in red skin were enriched in Kupffer’s vesicle development while the up-regulated DEGs in white skin were involved in cytoskeletal protein binding, sarcomere organization and glycogen phosphorylase activity. The distinct enriched activity might be associated with different structures and functions in erythrophores and iridophores. The DNA methylation levels of two selected DEGs inversely correlated with gene expression, indicating the participation of DNA methylation in the coloration. This expression characterization of red—white skin along with the accompanying transcriptome-wide expression data will be a useful resource for further studies of pigment cell biology. View Full-Text
Keywords: pigmentation; transcriptome sequencing; DNA methylation; teleost pigmentation; transcriptome sequencing; DNA methylation; teleost
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, X.-M.; Song, Y.-N.; Xiao, G.-B.; Zhu, B.-H.; Xu, G.-C.; Sun, M.-Y.; Xiao, J.; Mahboob, S.; Al-Ghanim, K.A.; Sun, X.-W.; Li, J.-T. Gene Expression Variations of Red—White Skin Coloration in Common Carp (Cyprinus carpio). Int. J. Mol. Sci. 2015, 16, 21310-21329.

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