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Int. J. Mol. Sci. 2015, 16(8), 16763-16777; doi:10.3390/ijms160816763

Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis

Institute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu 300, Taiwan
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Author to whom correspondence should be addressed.
Academic Editor: Fan-Gang Tseng
Received: 1 June 2015 / Revised: 7 July 2015 / Accepted: 17 July 2015 / Published: 23 July 2015
(This article belongs to the Special Issue Single Cell Analysis in Biotechnology and Systems Biology)
View Full-Text   |   Download PDF [992 KB, uploaded 23 July 2015]   |  

Abstract

This review describes the microfluidic techniques developed for the analysis of a single cell. The characteristics of microfluidic (e.g., little sample amount required, high-throughput performance) make this tool suitable to answer and to solve biological questions of interest about a single cell. This review aims to introduce microfluidic related techniques for the isolation, trapping and manipulation of a single cell. The major approaches for detection in single-cell analysis are introduced; the applications of single-cell analysis are then summarized. The review concludes with discussions of the future directions and opportunities of microfluidic systems applied in analysis of a single cell. View Full-Text
Keywords: microfluidic techniques; single-cell manipulation; single-cell analysis microfluidic techniques; single-cell manipulation; single-cell analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lo, S.-J.; Yao, D.-J. Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis. Int. J. Mol. Sci. 2015, 16, 16763-16777.

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