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Open AccessCommunication
Int. J. Mol. Sci. 2015, 16(6), 14098-14108; doi:10.3390/ijms160614098

Expression Profiling of Circulating MicroRNAs in Canine Myxomatous Mitral Valve Disease

1
Nestle Purina Research, Saint Louis, MO 63164, USA
2
Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA
*
Author to whom correspondence should be addressed.
Academic Editor: H. W. M. Niessen
Received: 29 April 2015 / Revised: 4 June 2015 / Accepted: 9 June 2015 / Published: 19 June 2015
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
View Full-Text   |   Download PDF [896 KB, uploaded 19 June 2015]   |  

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that have shown promise as noninvasive biomarkers in cardiac disease. This study was undertaken to investigate the miRNA expression profile in dogs with myxomatous mitral valve disease (MMVD). 277 miRNAs were quantified using RT-qPCR from six normal dogs (American College of Veterinary Internal Medicine Stage A), six dogs with MMVD mild to moderate cardiac enlargement (ACVIM Stage B1/B2) and six dogs with MMVD and congestive heart failure (ACVIM Stage C/D). Eleven miRNAs were differentially expressed (False Discovery Rate < 0.05). Dogs in Stage B1/B2 or C/D had four upregulated miRNAs, including three cfa-let-7/cfa-miR-98 family members, while seven others were downregulated, compared to Stage A. Expression of six of the 11 miRNAs also were significantly different between dogs in Stage C/D and those in Stage B1/B2. The expression changes were greater as disease severity increased. These miRNAs may be candidates for novel biomarkers and may provide insights into genetic regulatory pathways in canine MMVD. View Full-Text
Keywords: microRNA; dog; congestive heart failure; biomarker; myxomatous mitral valve disease; RT-qPCR microRNA; dog; congestive heart failure; biomarker; myxomatous mitral valve disease; RT-qPCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, Q.; Freeman, L.M.; Rush, J.E.; Laflamme, D.P. Expression Profiling of Circulating MicroRNAs in Canine Myxomatous Mitral Valve Disease. Int. J. Mol. Sci. 2015, 16, 14098-14108.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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