Next Article in Journal
The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic Balance
Next Article in Special Issue
Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry
Previous Article in Journal
Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma
Previous Article in Special Issue
Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2015, 16(6), 12686-12712; doi:10.3390/ijms160612686

Single Cell Electrical Characterization Techniques

1
Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia
2
Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Fan-Gang Tseng
Received: 16 February 2015 / Accepted: 13 April 2015 / Published: 4 June 2015
(This article belongs to the Special Issue Single Cell Analysis in Biotechnology and Systems Biology)
View Full-Text   |   Download PDF [1345 KB, uploaded 4 June 2015]   |  

Abstract

Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell’s electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell’s electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed. View Full-Text
Keywords: conversional patch clamp; electrical properties; electrorotation; impedance flow cytometry; microelectrical impedance spectroscopy (µEIS); single cell analysis (SCA) conversional patch clamp; electrical properties; electrorotation; impedance flow cytometry; microelectrical impedance spectroscopy (µEIS); single cell analysis (SCA)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mansor, M.A.; Ahmad, M.R. Single Cell Electrical Characterization Techniques. Int. J. Mol. Sci. 2015, 16, 12686-12712.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top