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Int. J. Mol. Sci. 2015, 16(3), 5618-5634; doi:10.3390/ijms16035618

PEDF Improves Cardiac Function in Rats with Acute Myocardial Infarction via Inhibiting Vascular Permeability and Cardiomyocyte Apoptosis

1
Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China
2
Research Facility Center for Morphology, Xuzhou Medical College, Xuzhou 221004, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: H.W.M. Niessen
Received: 12 January 2015 / Revised: 25 February 2015 / Accepted: 5 March 2015 / Published: 11 March 2015
(This article belongs to the Special Issue Improvement of Cardiac Function in Heart Failure)
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Abstract

Pigment epithelium-derived factor (PEDF) is a pleiotropic gene with anti-inflammatory, antioxidant and anti-angiogenic properties. However, recent reports about the effects of PEDF on cardiomyocytes are controversial, and it is not known whether and how PEDF acts to inhibit hypoxic or ischemic endothelial injury in the heart. In the present study, adult Sprague-Dawley rat models of acute myocardial infarction (AMI) were surgically established. PEDF-small interfering RNA (siRNA)-lentivirus (PEDF-RNAi-LV) or PEDF-LV was delivered into the myocardium along the infarct border to knockdown or overexpress PEDF, respectively. Vascular permeability, cardiomyocyte apoptosis, myocardial infarct size and animal cardiac function were analyzed. We also evaluated PEDF’s effect on the suppression of the endothelial permeability and cardiomyocyte apoptosis under hypoxia in vitro. The results indicated that PEDF significantly suppressed the vascular permeability and inhibited hypoxia-induced endothelial permeability through PPARγ-dependent tight junction (TJ) production. PEDF protected cardiomyocytes against ischemia or hypoxia-induced cell apoptosis both in vivo and in vitro via preventing the activation of caspase-3. We also found that PEDF significantly reduced myocardial infarct size and enhanced cardiac function in rats with AMI. These data suggest that PEDF could protect cardiac function from ischemic injury, at least by means of reducing vascular permeability, cardiomyocyte apoptosis and myocardial infarct size. View Full-Text
Keywords: pigment epithelium-derived factor (PEDF); myocardial infarction; cardiac function; vascular permeability; PPARγ; apoptosis pigment epithelium-derived factor (PEDF); myocardial infarction; cardiac function; vascular permeability; PPARγ; apoptosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhang, H.; Wang, Z.; Feng, S.-J.; Xu, L.; Shi, H.-X.; Chen, L.-L.; Yuan, G.-D.; Yan, W.; Zhuang, W.; Zhang, Y.-Q.; Zhang, Z.-M.; Dong, H.-Y. PEDF Improves Cardiac Function in Rats with Acute Myocardial Infarction via Inhibiting Vascular Permeability and Cardiomyocyte Apoptosis. Int. J. Mol. Sci. 2015, 16, 5618-5634.

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