Next Article in Journal
Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis
Next Article in Special Issue
Effects of Heat Stress on Metabolite Accumulation and Composition, and Nutritional Properties of Durum Wheat Grain
Previous Article in Journal
A Combined Metabolomic and Proteomic Analysis of Gestational Diabetes Mellitus
Previous Article in Special Issue
Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(12), 30046-30060; doi:10.3390/ijms161226137

Biosynthesis of Essential Polyunsaturated Fatty Acids in Wheat Triggered by Expression of Artificial Gene

1
Research Institute of Plant Production, National Agricultural and Food Center, 921 68 Piešťany, Slovakia
2
Department of Biotechnology, Faculty of Natural Sciences, University of SS, Cyril and Methodius in Trnava, 917 01 Trnava, Slovakia
3
Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, 949 74 Nitra, Slovakia
4
Faculty of Chemical and Food Technology, Slovak University of Technology, 812 37 Bratislava, Slovakia
5
Cereal Research Non-profit Ltd., Szeged, Alsó kikötö sor 9, H-6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Ute Roessner
Received: 22 October 2015 / Revised: 16 November 2015 / Accepted: 19 November 2015 / Published: 16 December 2015
(This article belongs to the Special Issue Metabolomics in the Plant Sciences)
View Full-Text   |   Download PDF [3455 KB, uploaded 16 December 2015]   |  

Abstract

The artificial gene D6D encoding the enzyme ∆6desaturase was designed and synthesized using the sequence of the same gene from the fungus Thamnidium elegans. The original start codon was replaced by the signal sequence derived from the wheat gene for high-molecular-weight glutenin subunit and the codon usage was completely changed for optimal expression in wheat. Synthesized artificial D6D gene was delivered into plants of the spring wheat line CY-45 and the gene itself, as well as transcribed D6D mRNA were confirmed in plants of T0 and T1 generations. The desired product of the wheat genetic modification by artificial D6D gene was the γ-linolenic acid. Its presence was confirmed in mature grains of transgenic wheat plants in the amount 0.04%–0.32% (v/v) of the total amount of fatty acids. Both newly synthesized γ-linolenic acid and stearidonic acid have been detected also in leaves, stems, roots, awns, paleas, rachillas, and immature grains of the T1 generation as well as in immature and mature grains of the T2 generation. Contents of γ-linolenic acid and stearidonic acid varied in range 0%–1.40% (v/v) and 0%–1.53% (v/v) from the total amount of fatty acids, respectively. This approach has opened the pathway of desaturation of fatty acids and production of essential polyunsaturated fatty acids in wheat. View Full-Text
Keywords: wheat; essential fatty acid; γ-linolenic acid; ∆6desaturase; artificial gene; transgene wheat; essential fatty acid; γ-linolenic acid; 6desaturase; artificial gene; transgene
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mihálik, D.; Klčová, L.; Ondreičková, K.; Hudcovicová, M.; Gubišová, M.; Klempová, T.; Čertík, M.; Pauk, J.; Kraic, J. Biosynthesis of Essential Polyunsaturated Fatty Acids in Wheat Triggered by Expression of Artificial Gene. Int. J. Mol. Sci. 2015, 16, 30046-30060.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top