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Int. J. Mol. Sci. 2015, 16(12), 29911-29922; doi:10.3390/ijms161226206

Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women

1
Grup de Recerca GEMMAIR (AGAUR)—Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d’Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain
2
Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain
3
Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d’Investigació Pere Virgili (IISPV), Avinguda Doctor Josep Laporte, 2, 43204 Reus, Spain
4
Servei de Cirurgia, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Toshiro Arai
Received: 9 October 2015 / Revised: 13 November 2015 / Accepted: 4 December 2015 / Published: 16 December 2015
(This article belongs to the Special Issue Molecular Research on Obesity and Diabetes)
View Full-Text   |   Download PDF [1220 KB, uploaded 16 December 2015]   |  

Abstract

The purpose of this work was to evaluate the expression of fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot to analyze visceral (VAT) and subcutaneous (SAT) adipose tissue mRNA expression involved in de novo fatty acid synthesis (ACC1, FAS), fatty acid oxidation (PPARα, PPARδ) and inflammation (IL6, TNFα), in normal weight control women (BMI < 25 kg/m2, n = 35) and moderately obese women (BMI 30–38 kg/m2, n = 55). In SAT, ACC1, FAS and PPARα mRNA expression were significantly decreased in moderately obese women compared to controls. The downregulation reported in SAT was more pronounced when BMI increased. In VAT, lipogenic-related genes and PPARα were similar in both groups. Only PPARδ gene expression was significantly increased in moderately obese women. As far as inflammation is concerned, TNFα and IL6 were significantly increased in moderate obesity in both tissues. Our results indicate that there is a progressive downregulation in lipogenesis in SAT as BMI increases, which suggests that SAT decreases the synthesis of fatty acid de novo during the development of obesity, whereas in VAT lipogenesis remains active regardless of the degree of obesity. View Full-Text
Keywords: moderate obesity; fatty acid metabolism; adipose tissue; de novo fatty acid synthesis moderate obesity; fatty acid metabolism; adipose tissue; de novo fatty acid synthesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Guiu-Jurado, E.; Auguet, T.; Berlanga, A.; Aragonès, G.; Aguilar, C.; Sabench, F.; Armengol, S.; Porras, J.A.; Martí, A.; Jorba, R.; Hernández, M.; del Castillo, D.; Richart, C. Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women. Int. J. Mol. Sci. 2015, 16, 29911-29922.

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