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Int. J. Mol. Sci. 2015, 16(12), 29889-29899; doi:10.3390/ijms161226208

Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate

1
Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India
2
Department of Biotechnology, Kadi Sarva Vishwavidyalaya, II Floor, KBIPER Building, Kadi Campus, Sector-23, Gandhinagar-382023, Gujarat, India
3
Department of Biotechnology, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, India
4
Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, Milan 20133, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 16 November 2015 / Revised: 4 December 2015 / Accepted: 8 December 2015 / Published: 15 December 2015
(This article belongs to the Special Issue Molecular Research in Plant Secondary Metabolism 2015)
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Abstract

The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%–0.9% to 1.1%–1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants. View Full-Text
Keywords: elicitors; mevalonate pathway; steroidal saponins; 3-hydroxy-3-methylglutaryl-CoA reductase; sterol-3-β-glucosyl transferase; plant innate immunity elicitors; mevalonate pathway; steroidal saponins; 3-hydroxy-3-methylglutaryl-CoA reductase; sterol-3-β-glucosyl transferase; plant innate immunity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chaudhary, S.; Chikara, S.K.; Sharma, M.C.; Chaudhary, A.; Alam Syed, B.; Chaudhary, P.S.; Mehta, A.; Patel, M.; Ghosh, A.; Iriti, M. Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate. Int. J. Mol. Sci. 2015, 16, 29889-29899.

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