Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
AbstractThe effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%–0.9% to 1.1%–1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants. View Full-Text
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Chaudhary, S.; Chikara, S.K.; Sharma, M.C.; Chaudhary, A.; Alam Syed, B.; Chaudhary, P.S.; Mehta, A.; Patel, M.; Ghosh, A.; Iriti, M. Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate. Int. J. Mol. Sci. 2015, 16, 29889-29899.
Chaudhary S, Chikara SK, Sharma MC, Chaudhary A, Alam Syed B, Chaudhary PS, Mehta A, Patel M, Ghosh A, Iriti M. Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate. International Journal of Molecular Sciences. 2015; 16(12):29889-29899.Chicago/Turabian Style
Chaudhary, Spandan; Chikara, Surendra K.; Sharma, Mahesh C.; Chaudhary, Abhinav; Alam Syed, Bakhtiyar; Chaudhary, Pooja S.; Mehta, Aditya; Patel, Maulik; Ghosh, Arpita; Iriti, Marcello. 2015. "Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate." Int. J. Mol. Sci. 16, no. 12: 29889-29899.