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Int. J. Mol. Sci. 2015, 16(12), 28783-28799; doi:10.3390/ijms161226125

Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata

1
Department of Horticulture, Sunchon National University, Suncheon 540-950, Korea
2
Department of Plant Science, Seoul National University, Seoul 151-742, Korea
3
Department of Agricultural Education, Sunchon National University, Suncheon 540-950, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 27 October 2015 / Revised: 24 November 2015 / Accepted: 25 November 2015 / Published: 3 December 2015
(This article belongs to the Special Issue Plant Molecular Biology)
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Abstract

Ribosomal DNA (rDNA) of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer (IGS). The 45S rDNA of flowering plants includes the 5.8S, 18S and 25S rDNA genes, the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). This study identified six different types of 45S-IGS, A to F, which at 363 bp, 1121 bp, 1717 bp, 1969 bp, 2036 bp and 2111 bp in length, respectively, were much shorter than the reported reference IGS sequences in B. oleracea var. alboglabra. The shortest two IGS types, A and B, lacked the transcription initiation site, non-transcribed spacer, and external transcribed spacer. Functional behavior of those two IGS types in relation to rRNA synthesis is a subject of further investigation. The other four IGSs had subtle variations in the transcription termination site, guanine-cytosine (GC) content, and number of tandem repeats, but the external transcribed spacers of these four IGSs were quite similar in length. The 45S IGSs were found to follow Mendelian inheritance in a population of 15 F1s and their 30 inbred parental lines, which suggests that these sequences could be useful for development of new breeding tools. In addition, this study represents the first report of intra-specific (within subspecies) variation of the 45S IGS in B. oleracea. View Full-Text
Keywords: ribosomal DNA; intergenic spacer; 45S rDNA; Brassica oleracea var. capitata ribosomal DNA; intergenic spacer; 45S rDNA; Brassica oleracea var. capitata
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MDPI and ACS Style

Yang, K.; Robin, A.H.K.; Yi, G.-E.; Lee, J.; Chung, M.-Y.; Yang, T.-J.; Nou, I.-S. Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata. Int. J. Mol. Sci. 2015, 16, 28783-28799.

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