Int. J. Mol. Sci. 2015, 16(12), 28566-28581; doi:10.3390/ijms161226120
Parallel Reaction Monitoring: A Targeted Experiment Performed Using High Resolution and High Mass Accuracy Mass Spectrometry
1
W.M. Keck Foundation Biotechnology Resource Laboratory, School of Medicine, Yale University, 300 George Street, New Haven, CT 06511, USA
2
Department of Molecular Biophysics & Biochemistry, Yale University, New Haven, CT 06520, USA
Academic Editor: Laszlo Prokai
Received: 22 October 2015 / Revised: 24 November 2015 / Accepted: 25 November 2015 / Published: 2 December 2015
(This article belongs to the Special Issue Fourier Transform Mass Spectrometry in Molecular Sciences)
Abstract
The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review discusses the label-based and label-free methods of quantification that can be performed with the targeted approach. View Full-TextKeywords:
parallel reaction monitoring; PRM; selected reaction monitoring; SRM; proteomics; mass spectrometry; targeted; quantitative proteomics; Q-Exactive; high resolution; accurate mass
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Rauniyar, N. Parallel Reaction Monitoring: A Targeted Experiment Performed Using High Resolution and High Mass Accuracy Mass Spectrometry. Int. J. Mol. Sci. 2015, 16, 28566-28581.
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