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Int. J. Mol. Sci. 2015, 16(12), 28001-28013; doi:10.3390/ijms161226082

Adjuvant Immune Enhancement of Subunit Vaccine Encoding pSCPI of Streptococcus iniae in Channel Catfish (Ictalurus punctatus)

1
Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China
2
Department of Aquaculture, Rongchang Campus, Southwest University, Chongqing 402460, China
3
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China
4
Department of Aquaculture, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 26 October 2015 / Revised: 19 November 2015 / Accepted: 19 November 2015 / Published: 25 November 2015
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [6900 KB, uploaded 25 November 2015]   |  

Abstract

Channel catfish (Ictalurus punctatus) is an important agricultural fish that has been plagued by Streptococcus iniae (S. iniae) infections in recent years, some of them severe. C5a peptidase is an important virulent factor of S. iniae. In this study, the subunit vaccine containing the truncated part of C5a peptidase (pSCPI) was mixed with aluminum hydroxide gel (AH), propolis adjuvant (PA), and Freund’s Incomplete Adjuvant (FIA). The immunogenicity of the pSCPI was detected by Western-blot in vitro. The relative percent survival (RPS), lysozyme activity, antibody titers, and the expression of the related immune genes were monitored in vivo to evaluate the immune effects of the three different adjuvants. The results showed that pSCPI exerted moderate immune protection (RPS = 46.43%), whereas each of the three adjuvants improved the immune protection of pSCPI. The immunoprotection of pSCPI + AH, pSCPI + PA, and pSCPI + FIA was characterized by RPS values of 67.86%, 75.00% and, 85.71%, respectively. Further, each of the three different adjuvanted pSCPIs stimulated higher levels of lysozyme activity and antibody titers than the unadjuvanted pSCPI and/or PBS buffer. In addition, pSCPI + FIA and pSCPI + PA induced expression of the related immune genes under investigation, which was substantially higher than the levels stimulated by PBS. pSCPI + AH significantly stimulated the induction of MHC II β, CD4-L2, and IFN-γ, while it induced slightly higher production of TNF-α and even led to a decrease in the levels of IL-1β, MHC I α, and CD8 α. Therefore, we conclude that compared with the other two adjuvants, FIA combined with pSCPI is a more promising candidate adjuvant against S. iniae in channel catfish. View Full-Text
Keywords: Channel catfish; Streptococcus iniae; subunit vaccine; pSCPI; adjuvant; immune effect Channel catfish; Streptococcus iniae; subunit vaccine; pSCPI; adjuvant; immune effect
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MDPI and ACS Style

Jiang, J.; Zheng, Z.; Wang, K.; Wang, J.; He, Y.; Wang, E.; Chen, D.; Ouyang, P.; Geng, Y.; Huang, X. Adjuvant Immune Enhancement of Subunit Vaccine Encoding pSCPI of Streptococcus iniae in Channel Catfish (Ictalurus punctatus). Int. J. Mol. Sci. 2015, 16, 28001-28013.

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