MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e
AbstractAccumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3′UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7. View Full-Text
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Liu, Z.; Zhou, Y.; Yuan, Y.; Nie, F.; Peng, R.; Li, Q.; Lyu, Z.; Mao, Z.; Huang, L.; Zhou, L.; Li, Y.; Hao, J.; Ni, D.; Jin, Q.; Long, Y.; Ju, P.; Yu, W.; Liu, J.; Hu, Y.; Zhou, Q. MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e. Int. J. Mol. Sci. 2015, 16, 27945-27955.
Liu Z, Zhou Y, Yuan Y, Nie F, Peng R, Li Q, Lyu Z, Mao Z, Huang L, Zhou L, Li Y, Hao J, Ni D, Jin Q, Long Y, Ju P, Yu W, Liu J, Hu Y, Zhou Q. MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e. International Journal of Molecular Sciences. 2015; 16(11):27945-27955.Chicago/Turabian Style
Liu, Zhicheng; Zhou, Yuru; Yuan, Yue; Nie, Fang; Peng, Rui; Li, Qianyin; Lyu, Zhongshi; Mao, Zhaomin; Huang, Liyuan; Zhou, Li; Li, Yiman; Hao, Jing; Ni, Dongsheng; Jin, Qianni; Long, Yaoshui; Ju, Pan; Yu, Wen; Liu, Jianing; Hu, Yanxia; Zhou, Qin. 2015. "MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e." Int. J. Mol. Sci. 16, no. 11: 27945-27955.