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Int. J. Mol. Sci. 2015, 16(11), 27599-27608; doi:10.3390/ijms161126043

A Critical Role for Cysteine 57 in the Biological Functions of Selenium Binding Protein-1

1
Department of Pathology, Xinxiang Medical University, Xinxiang 453003, China
2
Department of Pathology, University of Illinois at Chicago, Chicago, IL 60612, USA
3
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Eva Freisinger
Received: 31 August 2015 / Revised: 19 October 2015 / Accepted: 10 November 2015 / Published: 18 November 2015
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

The concentration of selenium-binding protein1 (SBP1) is often lower in tumors than in the corresponding tissue and lower levels have been associated with poor clinical outcomes. SBP1 binds tightly selenium although what role selenium plays in its biological functions remains unknown. Previous studies indicated that cysteine 57 is the most likely candidate amino acid for selenium binding. In order to investigate the role of cysteine 57 in SBP1, this amino acid was altered to a glycine and the mutated protein was expressed in human cancer cells. The SBP1 half-life, as well as the cellular response to selenite cytotoxicity, was altered by this change. The ectopic expression of SBP1GLY also caused mitochondrial damage in HCT116 cells. Taken together, these results indicated that cysteine 57 is a critical determinant of SBP1 function and may play a significant role in mitochondrial function. View Full-Text
Keywords: selenium-binding protein1; glutathione peroxidase 1; selenite cytotoxicity; mitochondrial damage selenium-binding protein1; glutathione peroxidase 1; selenite cytotoxicity; mitochondrial damage
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ying, Q.; Ansong, E.; Diamond, A.M.; Yang, W. A Critical Role for Cysteine 57 in the Biological Functions of Selenium Binding Protein-1. Int. J. Mol. Sci. 2015, 16, 27599-27608.

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