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Int. J. Mol. Sci. 2015, 16(11), 26786-26796; doi:10.3390/ijms161125993

Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS

1
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea
2
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea
3
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 361-763, Korea
4
Theragen Etex, Suwon 443-270, Korea
5
Genomics Division, National Academy of Agricultural Science (NAAS), Jeonju 560-500, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Ute Roessner
Received: 25 August 2015 / Revised: 30 October 2015 / Accepted: 3 November 2015 / Published: 9 November 2015
(This article belongs to the Special Issue Metabolomics in the Plant Sciences)
View Full-Text   |   Download PDF [984 KB, uploaded 9 November 2015]   |  

Abstract

In this study, a method of metabolite profiling based on UPLC-QTOF/MS was developed to analyze Platycodon grandiflorum. In the optimal UPLC, various metabolites, including major platycosides, were separated well in 15 min. The metabolite extraction protocols were also optimized by selecting a solvent for use in the study, the ratio of solvent to sample and sonication time. This method was used to profile two different parts of P. grandiflorum, i.e., the roots of P. grandiflorum (PR) and the stems and leaves of P. grandiflorum (PS), in the positive and negative ion modes. As a result, PR and PS showed qualitatively and quantitatively different metabolite profiles. Furthermore, their metabolite compositions differed according to individual plant samples. These results indicate that the UPLC-QTOF/MS-based profiling method is a good tool to analyze various metabolites in P. grandiflorum. This metabolomics approach can also be applied to evaluate the overall quality of P. grandiflorum, as well as to discriminate the cultivars for the medicinal plant industry. View Full-Text
Keywords: Platycodon grandiflorum; metabolomics; UPLC-QTOF/MS Platycodon grandiflorum; metabolomics; UPLC-QTOF/MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lee, J.W.; Ji, S.-H.; Kim, G.-S.; Song, K.-S.; Um, Y.; Kim, O.T.; Lee, Y.; Hong, C.P.; Shin, D.-H.; Kim, C.-K.; Lee, S.-E.; Ahn, Y.-S.; Lee, D.-Y. Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS. Int. J. Mol. Sci. 2015, 16, 26786-26796.

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