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Int. J. Mol. Sci. 2015, 16(11), 25982-25998; doi:10.3390/ijms161125934

Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9)

1
Central Laboratory, Jilin University Second Hospital, Changchun 130041, China
2
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China
3
College of Life Sciences, Shenzhen University, Shenzhen 518057, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: David Sheehan
Received: 26 August 2015 / Revised: 13 October 2015 / Accepted: 22 October 2015 / Published: 30 October 2015
(This article belongs to the Collection Advances in Proteomic Research)
View Full-Text   |   Download PDF [1903 KB, uploaded 30 October 2015]   |  

Abstract

Avian influenza A viruses are serious veterinary pathogens that normally circulate among avian populations, causing substantial economic impacts. Some strains of avian influenza A viruses, such as H5N1, H9N2, and recently reported H7N9, have been occasionally found to adapt to humans from other species. In order to replicate efficiently in the new host, influenza viruses have to interact with a variety of host factors. In the present study, H7N9 nucleoprotein was transfected into human HEK293T cells, followed by immunoprecipitated and analyzed by proteomics approaches. A series of host proteins co-immunoprecipitated were identified with high confidence, some of which were found to be acetylated at their lysine residues. Bioinformatics analysis revealed that spliceosome might be the most relevant pathway involved in host response to nucleoprotein expression, increasing our emerging knowledge of host proteins that might be involved in influenza virus replication activities. View Full-Text
Keywords: nucleoprotein; influenza A virus; lysine acetylation; mass spectrometry; co-immunoprecipitation nucleoprotein; influenza A virus; lysine acetylation; mass spectrometry; co-immunoprecipitation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sun, N.; Sun, W.; Li, S.; Yang, J.; Yang, L.; Quan, G.; Gao, X.; Wang, Z.; Cheng, X.; Li, Z.; Peng, Q.; Liu, N. Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9). Int. J. Mol. Sci. 2015, 16, 25982-25998.

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