Int. J. Mol. Sci. 2014, 15(6), 10215-10232; doi:10.3390/ijms150610215
Article

Screening and Functional Analysis of the Peroxiredoxin Specifically Expressed in Bursaphelenchus xylophilus—The Causative Agent of Pine Wilt Disease

1email, 1,2email, 1email, 1email and 1,* email
Received: 21 April 2014; in revised form: 25 May 2014 / Accepted: 26 May 2014 / Published: 10 June 2014
(This article belongs to the collection Advances in Proteomic Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The pine wood nematode, Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. Accurately differentiating B. xylophilus from other nematodes species, especially its related species B. mucronatus, is important for pine wood nematode detection. Thus, we attempted to identify a specific protein in the pine wood nematode using proteomics technology. Here, we compared the proteomes of B. xylophilus and B. mucronatus using Two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization -time-of-flight/time-of-flight (MALDI-TOF/TOF-MS) technologies. In total, 15 highly expressed proteins were identified in B. xylophilus compared with B. mucronatus. Subsequently, the specificity of the proteins identified was confirmed by PCR using the genomic DNA of other nematode species. Finally, a gene encoding a specific protein (Bx-Prx) was obtained. This gene was cloned and expressed in E. coli. The in situ hybridisation pattern of Bx-Prx showed that it was expressed strongly in the tail of B. xylophilus. RNAi was used to assess the function of Bx-Prx, the results indicated that the gene was associated with the reproduction and pathogenicity of B. xylophilus. This discovery provides fundamental information for identifying B. xylophilus via a molecular approach. Moreover, the purified recombinant protein has potential as a candidate diagnostic antigen of pine wilt disease, which may lead to a new immunological detection method for the pine wood nematode.
Keywords: Bursaphelenchus xylophilus; comparative proteomics; 2-DE; in situ hybridization; prokaryotic expression
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MDPI and ACS Style

Fu, H.-Y.; Ren, J.-H.; Huang, L.; Li, H.; Ye, J.-R. Screening and Functional Analysis of the Peroxiredoxin Specifically Expressed in Bursaphelenchus xylophilus—The Causative Agent of Pine Wilt Disease. Int. J. Mol. Sci. 2014, 15, 10215-10232.

AMA Style

Fu H-Y, Ren J-H, Huang L, Li H, Ye J-R. Screening and Functional Analysis of the Peroxiredoxin Specifically Expressed in Bursaphelenchus xylophilus—The Causative Agent of Pine Wilt Disease. International Journal of Molecular Sciences. 2014; 15(6):10215-10232.

Chicago/Turabian Style

Fu, Han-Yu; Ren, Jia-Hong; Huang, Lin; Li, Hao; Ye, Jian-Ren. 2014. "Screening and Functional Analysis of the Peroxiredoxin Specifically Expressed in Bursaphelenchus xylophilus—The Causative Agent of Pine Wilt Disease." Int. J. Mol. Sci. 15, no. 6: 10215-10232.

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