Int. J. Mol. Sci. 2013, 14(7), 15092-15104; doi:10.3390/ijms140715092
Article

Targeted Silencing of MART-1 Gene Expression by RNA Interference Enhances the Migration Ability of Uveal Melanoma Cells

1 Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China 2 Department of Clinical Laboratories, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 15 May 2013; in revised form: 11 July 2013 / Accepted: 15 July 2013 / Published: 19 July 2013
PDF Full-text Download PDF Full-Text [1773 KB, uploaded 19 July 2013 12:06 CEST]
Abstract: Uveal melanoma (UM) is the most common primary intraocular malignancy and the leading potentially fatal primary intraocular disease in adults. Melanoma antigen recognized by T-cells (MART-1) has been studied extensively as a clinically important diagnostic marker for melanoma, however, its biological function remains unclear. In the present study, the UM cell line SP6.5, which showed a high level of MART-1 expression, was subjected to small interfering RNA-mediated silencing of MART-1. Silencing of MART-1 expression increased the migration ability of SP6.5 cells and down-regulated the expression of the metastasis suppressor NM23. Our results suggest that MART-1 is a candidate target for the development of therapeutic strategies for UM and in particular for the suppression of metastasis associated with this malignancy.
Keywords: uveal melanoma; MART-1; NM23; migration ability

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Zhang, Y.; Jia, R.; Wang, J.; Xu, X.; Yao, Y.; Ge, S.; Fan, X. Targeted Silencing of MART-1 Gene Expression by RNA Interference Enhances the Migration Ability of Uveal Melanoma Cells. Int. J. Mol. Sci. 2013, 14, 15092-15104.

AMA Style

Zhang Y, Jia R, Wang J, Xu X, Yao Y, Ge S, Fan X. Targeted Silencing of MART-1 Gene Expression by RNA Interference Enhances the Migration Ability of Uveal Melanoma Cells. International Journal of Molecular Sciences. 2013; 14(7):15092-15104.

Chicago/Turabian Style

Zhang, Yidan; Jia, Renbing; Wang, Jing; Xu, Xiaofang; Yao, Yuting; Ge, Shengfan; Fan, Xianqun. 2013. "Targeted Silencing of MART-1 Gene Expression by RNA Interference Enhances the Migration Ability of Uveal Melanoma Cells." Int. J. Mol. Sci. 14, no. 7: 15092-15104.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert