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Int. J. Mol. Sci. 2013, 14(7), 15092-15104; doi:10.3390/ijms140715092
Article

Targeted Silencing of MART-1 Gene Expression by RNA Interference Enhances the Migration Ability of Uveal Melanoma Cells

1,†
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1,†
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1,*  and 1,*
1 Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China 2 Department of Clinical Laboratories, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 15 May 2013 / Revised: 11 July 2013 / Accepted: 15 July 2013 / Published: 19 July 2013
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Abstract

Uveal melanoma (UM) is the most common primary intraocular malignancy and the leading potentially fatal primary intraocular disease in adults. Melanoma antigen recognized by T-cells (MART-1) has been studied extensively as a clinically important diagnostic marker for melanoma, however, its biological function remains unclear. In the present study, the UM cell line SP6.5, which showed a high level of MART-1 expression, was subjected to small interfering RNA-mediated silencing of MART-1. Silencing of MART-1 expression increased the migration ability of SP6.5 cells and down-regulated the expression of the metastasis suppressor NM23. Our results suggest that MART-1 is a candidate target for the development of therapeutic strategies for UM and in particular for the suppression of metastasis associated with this malignancy.
Keywords: uveal melanoma; MART-1; NM23; migration ability uveal melanoma; MART-1; NM23; migration ability
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Zhang, Y.; Jia, R.; Wang, J.; Xu, X.; Yao, Y.; Ge, S.; Fan, X. Targeted Silencing of MART-1 Gene Expression by RNA Interference Enhances the Migration Ability of Uveal Melanoma Cells. Int. J. Mol. Sci. 2013, 14, 15092-15104.

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