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Int. J. Mol. Sci. 2013, 14(7), 13129-13136; doi:10.3390/ijms140713129
Article

Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion

1,2,†
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 and 1,*
Received: 3 May 2013; in revised form: 3 June 2013 / Accepted: 7 June 2013 / Published: 25 June 2013
(This article belongs to the Special Issue Pathogenesis and Prevention of Colorectal Cancer)
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Abstract: The aim of our study was to evaluate the diagnosis of desmoplastic reaction (DR) by immunostaining for α-smooth muscle actin (αSMA) and desmin, for predicting the depth of submucosal invasion in biopsy specimens of early colorectal carcinomas (CRCs). Thirty-eight cases of non-pedunculated early CRCs were included in this study. Positive for DR was defined as αSMA-positive and desmin-negative stroma in the CRC. The depth of submucosal invasion was measured in endoscopically or surgically resected specimens and the lesions were subsequently divided into two groups: Group A (carcinoma in situ/intramucosal carcinoma and submucosal invasive carcinoma with a depth <1000 μm) and Group B (submucosal invasion with a depth ≥1000 μm). Twenty-one cases were DR-positive and 17 were DR-negative. No statistical significance was found between the DR with regard to tumor size, location and histological type. All DR-positive cases belonged to Group B whereas 14 (82.4%) DR-negative lesions belonged to Group A (p < 0.001). The sensitivity, specificity, positive and negative predictive values and accuracy of DR positivity for diagnosis of Group B were 87.5%, 100%, 100%, 82.4% and 92.1%, respectively. Conclusively, detection of DR in biopsy specimens with ancillary immunohistochemistry (αSMA/desmin) would help in preoperative diagnosis for the depth of submucosal invasion of early CRC.
Keywords: immunohistochemistry; biopsy; desmoplastic reaction; early colorectal carcinoma; submucosal invasion immunohistochemistry; biopsy; desmoplastic reaction; early colorectal carcinoma; submucosal invasion
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ohno, K.; Fujimori, T.; Okamoto, Y.; Ichikawa, K.; Yamaguchi, T.; Imura, J.; Tomita, S.; Mitomi, H. Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion. Int. J. Mol. Sci. 2013, 14, 13129-13136.

AMA Style

Ohno K, Fujimori T, Okamoto Y, Ichikawa K, Yamaguchi T, Imura J, Tomita S, Mitomi H. Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion. International Journal of Molecular Sciences. 2013; 14(7):13129-13136.

Chicago/Turabian Style

Ohno, Kazuya; Fujimori, Takahiro; Okamoto, Yosuke; Ichikawa, Kazuhito; Yamaguchi, Takeshi; Imura, Johji; Tomita, Shigeki; Mitomi, Hiroyuki. 2013. "Diagnosis of Desmoplastic Reaction by Immunohistochemical Analysis, in Biopsy Specimens of Early Colorectal Carcinomas, Is Efficacious in Estimating the Depth of Invasion." Int. J. Mol. Sci. 14, no. 7: 13129-13136.


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