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Int. J. Mol. Sci. 2013, 14(6), 11113-11124; doi:10.3390/ijms140611113
Article

The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques

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Received: 21 March 2013; in revised form: 2 May 2013 / Accepted: 16 May 2013 / Published: 24 May 2013
(This article belongs to the Special Issue Biofilms: Extracellular Bastions of Bacteria)
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Abstract: In order to investigate the microbe-mineral interaction in the micro scale, spatial distribution and speciation of Cu and S in Halothiobacillus HT1 biofilm formed on a CuS surface was examined using synchrotron-based X-ray techniques. Confocal laser scanning microscope (CLSM) results indicated that Halothiobacillus HT1 biofilm formation gave rise to distinct chemical and redox gradients, leading to diverse niches in the biofilm. Live cells were distributed at the air-biofilm and membrane-biofilm interface. CuS was oxidized by Halothiobacillus HT1 biofilm, and copper penetrated into the biofilm. Sulfide was oxidized to cysteine (77.3%), sulfite (3.8%) and sulfonate (18.9%). Cu-cysteine-like species were involved in the copper homeostasis. These results significantly improve our understanding of the interfacial properties of the biofilm-mineral interface.
Keywords: Halothiobacillus HT1 biofilm; distribution; speciation; copper; sulfur Halothiobacillus HT1 biofilm; distribution; speciation; copper; sulfur
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lin, H.; Chen, G.; Zhu, S.; Chen, Y.; Chen, D.; Xu, W.; Yu, X.; Shi, J. The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques. Int. J. Mol. Sci. 2013, 14, 11113-11124.

AMA Style

Lin H, Chen G, Zhu S, Chen Y, Chen D, Xu W, Yu X, Shi J. The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques. International Journal of Molecular Sciences. 2013; 14(6):11113-11124.

Chicago/Turabian Style

Lin, Huirong; Chen, Guangcun; Zhu, Shenhai; Chen, Yingxu; Chen, Dongliang; Xu, Wei; Yu, Xiaohan; Shi, Jiyan. 2013. "The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques." Int. J. Mol. Sci. 14, no. 6: 11113-11124.


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