Secretory Phospholipases A₂ in Durum Wheat (Triticum durum Desf.): Gene Expression, Enzymatic Activity, and Relation to Drought Stress Adaptation

Abstract: Phospholipases A₂ (PLA₂s) are known to mediate signaling cascades during plant growth and development, as well as biotic and abiotic stress responses. In this context, the present study provides extensive characterization of specific PLA₂s in durum wheat, and assesses their involvement in durum wheat response to drought stress. In durum wheat leaves, four full-length expressed sequences encoding putative PLA₂s were isolated and characterized as belonging to the class of secretory PLA₂s (sPLA₂s): TdsPLA₂I, TdsPLA₂II, TdsPLA₂III and TdsPLA₂IV. PLA₂ activity was also detected, the characteristics of which resemble those of previously characterized plant sPLA₂s: strong preference for phospholipids; requirement for millimolar Ca²⁺ concentrations; optimal activity at basic pH; heat stability; and inhibition by the reducing agent dithiothreitol. With drought stress imposed at both the vegetative and reproductive stages, accumulation of TdsPLA₂I and TdsPLA₂III transcripts, and to a lesser extent of TdsPLA₂IV transcript, paralleled increased PLA₂ activity; both transcript levels and enzymatic activity decreased as a consequence of stress recovery. Consistently, free fatty acid analysis of drought-stressed leaves revealed increased linoleate, linolenate and palmitate contents, which were reversed by plant re-watering. Overall, these findings strongly suggest that there are inducible sPLA₂ isoforms in durum wheat that have roles in orchestrating the plant response to drought stress.