Int. J. Mol. Sci. 2013, 14(12), 23441-23453; doi:10.3390/ijms141223441
Article

Bone Morphogenetic Protein-7 Ameliorates Cerebral Ischemia and Reperfusion Injury via Inhibiting Oxidative Stress and Neuronal Apoptosis

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Received: 21 September 2013; in revised form: 12 November 2013 / Accepted: 19 November 2013 / Published: 28 November 2013
(This article belongs to the collection Programmed Cell Death and Apoptosis)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Previous studies have indicated that bone morphogenetic protein-7 (BMP-7) is neuroprotective against cerebral ischemia/reperfusion (IR) injury. The present study was undertaken to determine the molecular mechanisms involved in this effect. Adult male Wistar rats were subjected to 2 h of transient middle cerebral artery occlusion (MCAO), followed by 24 h of reperfusion. BMP-7 (10−4 g/kg) or vehicle was infused into rats at the onset of reperfusion via the tail vein. Neurological deficits, infarct volume, histopathological changes, oxidative stress-related biochemical parameters, neuronal apoptosis, and apoptosis-related proteins were assessed. BMP-7 significantly improved neurological and histological deficits, reduced the infarct volume, and decreased apoptotic cells after cerebral ischemia. BMP-7 also markedly enhanced the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and reduced the level of malondialdehyde (MDA) in IR rats. In addition, Western blot analysis indicated that BMP-7 prevented cytochrome c release, inhibited activation of caspase-3, caspase-9 and caspase-8. Our data suggested that BMP-7 has protective effects against cerebral IR injury in rats, and the neuroprotective effects may be attributed to attenuating oxidative stress and inhibiting neuronal apoptosis.
Keywords: bone morphogenetic protein-7; cerebral ischemia/reperfusion injury; oxidative stress; apoptosis
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MDPI and ACS Style

Pei, H.; Cao, D.; Guo, Z.; Liu, G.; Guo, Y.; Lu, C. Bone Morphogenetic Protein-7 Ameliorates Cerebral Ischemia and Reperfusion Injury via Inhibiting Oxidative Stress and Neuronal Apoptosis. Int. J. Mol. Sci. 2013, 14, 23441-23453.

AMA Style

Pei H, Cao D, Guo Z, Liu G, Guo Y, Lu C. Bone Morphogenetic Protein-7 Ameliorates Cerebral Ischemia and Reperfusion Injury via Inhibiting Oxidative Stress and Neuronal Apoptosis. International Journal of Molecular Sciences. 2013; 14(12):23441-23453.

Chicago/Turabian Style

Pei, Haitao; Cao, Dongming; Guo, Zhuangli; Liu, Guofang; Guo, Yunliang; Lu, Chenglong. 2013. "Bone Morphogenetic Protein-7 Ameliorates Cerebral Ischemia and Reperfusion Injury via Inhibiting Oxidative Stress and Neuronal Apoptosis." Int. J. Mol. Sci. 14, no. 12: 23441-23453.

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