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Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Animal, Plant & Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 200001, China
* Author to whom correspondence should be addressed.
Received: 10 July 2013; in revised form: 6 September 2013 / Accepted: 12 September 2013 / Published: 25 September 2013
Abstract: It is challenging to detect 7-aminonitrazepam (7-ANZP) residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA) method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD) of 0.18 µg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.
Keywords: 7-aminonitrazepam; ELISA; gold nanoparticle; bioconjugate
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Cite This Article
MDPI and ACS Style
Peng, C.; Duan, X.; Song, S.; Xue, F. Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA. Int. J. Mol. Sci. 2013, 14, 19474-19483.
Peng C, Duan X, Song S, Xue F. Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA. International Journal of Molecular Sciences. 2013; 14(10):19474-19483.
Peng, Chifang; Duan, Xiaohui; Song, Shanshan; Xue, Feng. 2013. "Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA." Int. J. Mol. Sci. 14, no. 10: 19474-19483.