Int. J. Mol. Sci. 2013, 14(10), 19474-19483; doi:10.3390/ijms141019474
Article

Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA

1,* email, 1email, 1email and 2email
Received: 10 July 2013; in revised form: 6 September 2013 / Accepted: 12 September 2013 / Published: 25 September 2013
(This article belongs to the Section Molecular Pathology)
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Abstract: It is challenging to detect 7-aminonitrazepam (7-ANZP) residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA) method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD) of 0.18 µg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.
Keywords: 7-aminonitrazepam; ELISA; gold nanoparticle; bioconjugate
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Peng, C.; Duan, X.; Song, S.; Xue, F. Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA. Int. J. Mol. Sci. 2013, 14, 19474-19483.

AMA Style

Peng C, Duan X, Song S, Xue F. Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA. International Journal of Molecular Sciences. 2013; 14(10):19474-19483.

Chicago/Turabian Style

Peng, Chifang; Duan, Xiaohui; Song, Shanshan; Xue, Feng. 2013. "Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA." Int. J. Mol. Sci. 14, no. 10: 19474-19483.


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