Int. J. Mol. Sci. 2012, 13(7), 8970-8986; doi:10.3390/ijms13078970
Article

Enrichment and Purification of Syringin, Eleutheroside E and Isofraxidin from Acanthopanax senticosus by Macroporous Resin

Fengjian Yangemail, Lei Yang* email, Wenjie Wangemail, Yang Liuemail, Chunjian Zhaoemail and Yuangang Zu* email
State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China
* Authors to whom correspondence should be addressed.
Received: 30 May 2012; in revised form: 29 June 2012 / Accepted: 6 July 2012 / Published: 18 July 2012
(This article belongs to the Section Material Sciences and Nanotechnology)
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Abstract: In order to screen a suitable resin for the preparative simultaneous separation and purification of syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus, the adsorption and desorption properties of 17 widely used commercial macroporous resins were evaluated. According to our results, HPD100C, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for syringin, eleutheroside E and isofraxidin than other resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions were as follows: for adsorption, processing volume: 24 BV, flow rate: 2 BV/h; for desorption, ethanol–water solution: 60:40 (v/v), eluent volume: 4 BV, flow rate: 3 BV/h. Under the above conditions, the contents of syringin, eleutheroside E and isofraxidin increased 174-fold, 20-fold and 5-fold and their recoveries were 80.93%, 93.97% and 93.79%, respectively.
Keywords: Radix Acanthopanax senticosus; macroporous resin; syringin; eleutheroside E; isofraxidin

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MDPI and ACS Style

Yang, F.; Yang, L.; Wang, W.; Liu, Y.; Zhao, C.; Zu, Y. Enrichment and Purification of Syringin, Eleutheroside E and Isofraxidin from Acanthopanax senticosus by Macroporous Resin. Int. J. Mol. Sci. 2012, 13, 8970-8986.

AMA Style

Yang F, Yang L, Wang W, Liu Y, Zhao C, Zu Y. Enrichment and Purification of Syringin, Eleutheroside E and Isofraxidin from Acanthopanax senticosus by Macroporous Resin. International Journal of Molecular Sciences. 2012; 13(7):8970-8986.

Chicago/Turabian Style

Yang, Fengjian; Yang, Lei; Wang, Wenjie; Liu, Yang; Zhao, Chunjian; Zu, Yuangang. 2012. "Enrichment and Purification of Syringin, Eleutheroside E and Isofraxidin from Acanthopanax senticosus by Macroporous Resin." Int. J. Mol. Sci. 13, no. 7: 8970-8986.

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