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Int. J. Mol. Sci. 2012, 13(6), 7648-7662; doi:10.3390/ijms13067648
Article

Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

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Received: 10 May 2012; in revised form: 30 May 2012 / Accepted: 8 June 2012 / Published: 21 June 2012
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Abstract: Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold.
Keywords: Eucalyptus globulus; bark; triterpenoids; triterpenic acids; supercritical fluid extraction; carbon dioxide; biorefinery Eucalyptus globulus; bark; triterpenoids; triterpenic acids; supercritical fluid extraction; carbon dioxide; biorefinery
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Domingues, R.M.A.; Oliveira, E.L.G.; Freire, C.S.R.; Couto, R.M.; Simões, P.C.; Neto, C.P.; Silvestre, A.J.D.; Silva, C.M. Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production. Int. J. Mol. Sci. 2012, 13, 7648-7662.

AMA Style

Domingues RMA, Oliveira ELG, Freire CSR, Couto RM, Simões PC, Neto CP, Silvestre AJD, Silva CM. Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production. International Journal of Molecular Sciences. 2012; 13(6):7648-7662.

Chicago/Turabian Style

Domingues, Rui M. A.; Oliveira, Eduardo L. G.; Freire, Carmen S. R.; Couto, Ricardo M.; Simões, Pedro C.; Neto, Carlos P.; Silvestre, Armando J. D.; Silva, Carlos M. 2012. "Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production." Int. J. Mol. Sci. 13, no. 6: 7648-7662.



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