Next Article in Journal
Expression of Sox2 and Oct4 and Their Clinical Significance in Human Non-Small-Cell Lung Cancer
Previous Article in Journal
WIP Remodeling Actin behind the Scenes: How WIP Reshapes Immune and Other Functions
Int. J. Mol. Sci. 2012, 13(6), 7648-7662; doi:10.3390/ijms13067648
Article

Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

1
,
1
,
1
,
2
,
2
,
1
,
1
 and
1,*
1 CICECO, Department of Chemistry, University of Aveiro, Aveiro, 3810-193, Portugal 2 REQUIMTE, Department of Chemistry, Faculty of Science and Technology, New University of Lisbon, Caparica, 2829-516, Portugal
* Author to whom correspondence should be addressed.
Received: 10 May 2012 / Revised: 30 May 2012 / Accepted: 8 June 2012 / Published: 21 June 2012
View Full-Text   |   Download PDF [258 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold.
Keywords: Eucalyptus globulus; bark; triterpenoids; triterpenic acids; supercritical fluid extraction; carbon dioxide; biorefinery Eucalyptus globulus; bark; triterpenoids; triterpenic acids; supercritical fluid extraction; carbon dioxide; biorefinery
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Domingues, R.M.A.; Oliveira, E.L.G.; Freire, C.S.R.; Couto, R.M.; Simões, P.C.; Neto, C.P.; Silvestre, A.J.D.; Silva, C.M. Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production. Int. J. Mol. Sci. 2012, 13, 7648-7662.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert