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Int. J. Mol. Sci. 2012, 13(5), 6292-6302; doi:10.3390/ijms13056292
Article

Optimization of the Extraction of Anthocyanins from the Fruit Skin of Rhodomyrtus tomentosa (Ait.) Hassk and Identification of Anthocyanins in the Extract Using High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (HPLC-ESI-MS)

1,2
, 1,*  and 2
1 Department of Food Science, Yingdong College of Bioengineering, Shaoguan University, Shaoguan 512005, China 2 College of Food, South China Agricultural University, Guangzhou 51000, China
* Author to whom correspondence should be addressed.
Received: 13 March 2012 / Revised: 24 April 2012 / Accepted: 17 May 2012 / Published: 22 May 2012
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Abstract

Anthocyanins are naturally occurring polyphenols that impart bright color to fruits, vegetables and plants. In this study, the extraction of anthocyanins from freeze-dried fruit skin of downy rose-myrtle (Rhodomyrtus tomentosa (Ait.) Hassk var. Gangren) was optimized using response surface methodology (RSM). Using 60% ethanol containing 0.1% (v/v) hydrochloric acid as extraction solvent, the optimal conditions for maximum yields of anthocyanin (4.358 ± 0.045 mg/g) were 15.7:1 (v/w) liquid to solid ratio, 64.38 °C with a 116.88 min extraction time. The results showed good fits with the proposed model for the anthocyanin extraction (R2 = 0.9944). Furthermore, the results of high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) analysis of the anthocyanins extracted from the fruit skin of downy rose-myrtle revealed the presence of five anthocyanin components, which were tentatively identified as delphinidin-3-glucoside, cyanidin-3-glucoside, peonidin-3-glucoside, petunidin-3-glucoside and malvidin-3-glucoside.
Keywords: anthocyanins; Rhodomyrtus tomentosa (Ait.) Hassk; response surface methodology; HPLC-ESI-MS anthocyanins; Rhodomyrtus tomentosa (Ait.) Hassk; response surface methodology; HPLC-ESI-MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Liu, G.-L.; Guo, H.-H.; Sun, Y.-M. Optimization of the Extraction of Anthocyanins from the Fruit Skin of Rhodomyrtus tomentosa (Ait.) Hassk and Identification of Anthocyanins in the Extract Using High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (HPLC-ESI-MS). Int. J. Mol. Sci. 2012, 13, 6292-6302.

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