Int. J. Mol. Sci. 2012, 13(5), 5454-5467; doi:10.3390/ijms13055454
Article

Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia

Leandro J. Lizcano 1 email, María Viloria-Bernal 2 email, Francisca Vicente 2 email, Luis Angel Berrueta 2 email, Blanca Gallo 2 email, Magdalena Martínez-Cañamero 3 email, Maria Begoña Ruiz-Larrea 1,* email and José Ignacio Ruiz-Sanz 1 email
1 Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain 2 Department of Analytical Chemistry, Sciences and Technology School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain 3 Department of Health Sciences, Microbiology Area, Experimental Sciences School, University of Jaen, 23071-Jaen, Spain
* Author to whom correspondence should be addressed.
Received: 1 April 2012; in revised form: 23 April 2012 / Accepted: 2 May 2012 / Published: 4 May 2012
(This article belongs to the Special Issue Advances in Free Radicals in Biology and Medicine)
PDF Full-text Download PDF Full-Text [347 KB, uploaded 4 May 2012 10:48 CEST]
Abstract: Diverse plants of ethnobotanic interest in Amazonia are commonly used in traditional medicine. We determined the antioxidant potential against lipid peroxidation, the antimicrobial activity, and the polyphenol composition of several Amazonian plants (Brownea rosademonte, Piper glandulosissimum, Piper krukoffii, Piper putumayoense, Solanum grandiflorum, and Vismia baccifera). Extracts from the plant leaf, bark, and stem were prepared as aqueous infusions, as used in folk medicine, and added to rat liver microsomes exposed to iron. The polyphenolic composition was detected by reverse-phase HPLC coupled to diode-array detector and MS/MS analysis. The antimicrobial activity was tested by the spot-on-a-lawn method against several indicator microorganisms. All the extracts inhibited lipid oxidation, except the P. glandulosissimum stem. The plant extracts exhibiting high antioxidant potential (V. baccifera and B. rosademonte) contained high levels of flavanols (particularly, catechin and epicatechin). By contrast, S. grandiflorum leaf, which exhibited very low antioxidant activity, was rich in hydroxycinnamic acids. None of the extracts showed antimicrobial activity. This study demonstrates for the first time the presence of bioactive polyphenolic compounds in several Amazonian plants, and highlights the importance of flavanols as major phenolic contributors to antioxidant activity.
Keywords: polyphenols; lipid peroxidation; liver microsomes; HPLC-DAD-MS/MS; Amazonian plants

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Lizcano, L.J.; Viloria-Bernal, M.; Vicente, F.; Berrueta, L.A.; Gallo, B.; Martínez-Cañamero, M.; Ruiz-Larrea, M.B.; Ruiz-Sanz, J.I. Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia. Int. J. Mol. Sci. 2012, 13, 5454-5467.

AMA Style

Lizcano LJ, Viloria-Bernal M, Vicente F, Berrueta LA, Gallo B, Martínez-Cañamero M, Ruiz-Larrea MB, Ruiz-Sanz JI. Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia. International Journal of Molecular Sciences. 2012; 13(5):5454-5467.

Chicago/Turabian Style

Lizcano, Leandro J.; Viloria-Bernal, María; Vicente, Francisca; Berrueta, Luis Angel; Gallo, Blanca; Martínez-Cañamero, Magdalena; Ruiz-Larrea, Maria Begoña; Ruiz-Sanz, José Ignacio. 2012. "Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia." Int. J. Mol. Sci. 13, no. 5: 5454-5467.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert