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Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia
Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain
Department of Analytical Chemistry, Sciences and Technology School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain
Department of Health Sciences, Microbiology Area, Experimental Sciences School, University of Jaen, 23071-Jaen, Spain
* Author to whom correspondence should be addressed.
Received: 1 April 2012; in revised form: 23 April 2012 / Accepted: 2 May 2012 / Published: 4 May 2012
Abstract: Diverse plants of ethnobotanic interest in Amazonia are commonly used in traditional medicine. We determined the antioxidant potential against lipid peroxidation, the antimicrobial activity, and the polyphenol composition of several Amazonian plants (Brownea rosademonte, Piper glandulosissimum, Piper krukoffii, Piper putumayoense, Solanum grandiflorum, and Vismia baccifera). Extracts from the plant leaf, bark, and stem were prepared as aqueous infusions, as used in folk medicine, and added to rat liver microsomes exposed to iron. The polyphenolic composition was detected by reverse-phase HPLC coupled to diode-array detector and MS/MS analysis. The antimicrobial activity was tested by the spot-on-a-lawn method against several indicator microorganisms. All the extracts inhibited lipid oxidation, except the P. glandulosissimum stem. The plant extracts exhibiting high antioxidant potential (V. baccifera and B. rosademonte) contained high levels of flavanols (particularly, catechin and epicatechin). By contrast, S. grandiflorum leaf, which exhibited very low antioxidant activity, was rich in hydroxycinnamic acids. None of the extracts showed antimicrobial activity. This study demonstrates for the first time the presence of bioactive polyphenolic compounds in several Amazonian plants, and highlights the importance of flavanols as major phenolic contributors to antioxidant activity.
Keywords: polyphenols; lipid peroxidation; liver microsomes; HPLC-DAD-MS/MS; Amazonian plants
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Lizcano, L.J.; Viloria-Bernal, M.; Vicente, F.; Berrueta, L.A.; Gallo, B.; Martínez-Cañamero, M.; Ruiz-Larrea, M.B.; Ruiz-Sanz, J.I. Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia. Int. J. Mol. Sci. 2012, 13, 5454-5467.
Lizcano LJ, Viloria-Bernal M, Vicente F, Berrueta LA, Gallo B, Martínez-Cañamero M, Ruiz-Larrea MB, Ruiz-Sanz JI. Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia. International Journal of Molecular Sciences. 2012; 13(5):5454-5467.
Lizcano, Leandro J.; Viloria-Bernal, María; Vicente, Francisca; Berrueta, Luis Angel; Gallo, Blanca; Martínez-Cañamero, Magdalena; Ruiz-Larrea, Maria Begoña; Ruiz-Sanz, José Ignacio. 2012. "Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia." Int. J. Mol. Sci. 13, no. 5: 5454-5467.