Salivary Proteins Associated with Periodontitis in Patients with Type 2 Diabetes Mellitus
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Collection and Pre-Treatment of the Saliva Samples
3.2. First Dimensional Separation—Isoelectric Focusing
3.3. Second Dimensional Separation—Polyacrylamide Gel Electrophoresis
3.4. Scanning and Analysis of Gel Image
3.5. Identification of Saliva Proteins
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
- Conflict of InterestThe authors declare no conflict of interest.
References
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Proteins | Protein Label | Diabetes with Periodontitis % Volume a (±SEM) | RP b/15 | Diabetes with Healthy Periodontium % Volume a (±SEM) | RP b//10 |
---|---|---|---|---|---|
Serotransferrin | 1 | 2.602 (±1.274) | 15 | 3.430 (±1.254) | 10 |
pIgR | 2 | 5.093 (±1.591) | 15 | 6.848 (±1.273) | 10 |
Plastin 2 | 4 | 0.434 (±0.161) | 15 | 0.182 (±0.039) | 8 |
Alpha-1-antitrypsin | 6 | 1.858 (±0.641) | 15 | 1.818 (±0.836) | 10 |
Protein disulfide isomerase | 7 | 0.230 (±0.090) | 14 | 0.199 (±0.093) | 8 |
Actin-related protein 3 | 8 | 0.023 (±0.004) | 14 | 0.135 (±0.088) | 9 |
Zinc-alpha-2-glycoprotein | 9 | 3.816 (±1.236) | 15 | 3.735 (±0.671) | 10 |
11 | |||||
12 | |||||
13 | |||||
Actin beta | 10 | 1.567 (±0.584) | 15 | 1.913 (±0.883) | 10 |
16 | |||||
17 | |||||
Leukocyte elastase inhibitor | 14 | 0.386 (±0.162) | 14 | 0.201 (±0.126) | 9 |
Carbonic anhydrase VI | 15 | 1.665 (±0.595) | 14 | 2.273 (±0.549) | 10 |
Complement C3 | 18 | 0.582 (±0.278) | 15 | 0.626 (±0.268) | 10 |
SPLUNC | 19 | 2.193 (±0.962) | 12 | 1.117 (±0.776) | 9 |
20 | |||||
21 | |||||
Annexin A3 | 22 | 0.199 (±0.098) | 15 | 0.133 (±0.091) | 8 |
Ig Kappa chain c region | 23 | 23.031 (±3.968) | 15 | 17.870 (±8.369) | 10 |
Immunoglobulin J chain | 24 | 2.402 (±0.338) | 15 | 1.378 (±1.706) | 10 |
Glutathione S Transferase | 25 | 0.369 (±0.252) | 15 | 0.270 (±0.112) | 10 |
Prolactin inducible protein | 26 | 6.362 (±2.176) | 15 | 4.648 (±1.388) | 10 |
27 | |||||
28 | |||||
32 | |||||
33 | |||||
34 | |||||
Lipocalin -1 | 29 | 1.948 (±0.926) | 15 | 1.602 (±0.455) | 10 |
30 | |||||
31 | |||||
35 | |||||
37 | |||||
Interleukin-1 receptor antagonist protein | 36 | 0.041 (±0.016) | 15 | 0.171 (±0.060) | 9 |
Haptoglobin | 38 | 0.397 (±0.246) | 15 | 0.578 (±0.323) | 10 |
39 | |||||
40 | |||||
Transthyretin | 41 | 0.213 (±0.125) | 13 | 0.215 (±0.145) | 5 |
42 |
Proteins | Fold Change a | p-Value |
---|---|---|
PIGR | −1.344 | 0.008 |
Plastin 2 | +2.381 | 0 |
Actin-related protein 3 | −5.802 | 0.001 |
Leukocyte elastase inhibitor | +1.919 | 0.004 |
Carbonic anhydrase VI | −1.365 | 0.012 |
Immunoglobulin J chain | +1.743 | 0 |
Interleukin-1 receptor antagonist | −4.132 | 0 |
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Chan, H.H.; Rahim, Z.H.A.; Jessie, K.; Hashim, O.H.; Taiyeb-Ali, T.B. Salivary Proteins Associated with Periodontitis in Patients with Type 2 Diabetes Mellitus. Int. J. Mol. Sci. 2012, 13, 4642-4654. https://doi.org/10.3390/ijms13044642
Chan HH, Rahim ZHA, Jessie K, Hashim OH, Taiyeb-Ali TB. Salivary Proteins Associated with Periodontitis in Patients with Type 2 Diabetes Mellitus. International Journal of Molecular Sciences. 2012; 13(4):4642-4654. https://doi.org/10.3390/ijms13044642
Chicago/Turabian StyleChan, Hang Haw, Zubaidah H. A. Rahim, Kala Jessie, Onn H. Hashim, and Tara B. Taiyeb-Ali. 2012. "Salivary Proteins Associated with Periodontitis in Patients with Type 2 Diabetes Mellitus" International Journal of Molecular Sciences 13, no. 4: 4642-4654. https://doi.org/10.3390/ijms13044642