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Int. J. Mol. Sci. 2012, 13(4), 4153-4167; doi:10.3390/ijms13044153

Labeling of Anti-MUC-1 Binding Single Chain Fv Fragments to Surface Modified Upconversion Nanoparticles for an Initial in Vivo Molecular Imaging Proof of Principle Approach

1
Department of Chemistry, University of Osnabrück, Barbarastr. 7, 46069 Osnabrück, Germany
2
Philips Research Laboratories Aachen, Weisshausstr. 2, 52066 Aachen, Germany
3
Frauenhofer IME, Fockenbeckstr. 6, 52074 Aachen, Germany
4
Bayer Technology Services GmbH, Building E41, 51368 Leverkusen, Germany
5
Molecular Imaging Group, Department of Clinical Radiology, University Hospital Münster, Waldeyerstr. 1, 48149 Münster, Germany
*
Author to whom correspondence should be addressed.
Received: 22 February 2012 / Revised: 7 March 2012 / Accepted: 19 March 2012 / Published: 29 March 2012
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Abstract

In vivo optical Imaging is an inexpensive and highly sensitive modality to investigate and follow up diseases like breast cancer. However, fluorescence labels and specific tracers are still works in progress to bring this promising modality into the clinical day-to-day use. In this study an anti-MUC-1 binding single-chain antibody fragment was screened, produced and afterwards labeled with newly designed and surface modified NaYF4:Yb,Er upconversion nanoparticles as fluorescence reporter constructs. The MUC-1 binding of the conjugate was examined in vitro and in vivo using modified state-of-the-art small animal Imaging equipment. Binding of the newly generated upconversion nanoparticle based probe to MUC-1 positive cells was clearly shown via laser scanning microscopy and in an initial proof of principal small animal optical imaging approach. View Full-Text
Keywords: anti-MUC-1 single-chain antibody fragment; upconversion nanoparticles; labeling; in vivo optical molecular imaging anti-MUC-1 single-chain antibody fragment; upconversion nanoparticles; labeling; in vivo optical molecular imaging
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hischemöller, A.; Walter, C.; Weiler, V.; Hummel, H.; Thepen, T.; Huhn, M.; Barth, S.; Hoheisel, W.; Köhler, K.; Dimova-Landen, D.; Bremer, C.; Haase, M.; Waldeck, J. Labeling of Anti-MUC-1 Binding Single Chain Fv Fragments to Surface Modified Upconversion Nanoparticles for an Initial in Vivo Molecular Imaging Proof of Principle Approach. Int. J. Mol. Sci. 2012, 13, 4153-4167.

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