Abstract: In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 μg/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys3 and Cys9 or Cys10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents.
Keywords: AFP-J; partial acid digestion; Potide-J; antibiotic agent
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Lee, J.-K.; Gopal, R.; Seo, C.H.; Cheong, H.; Park, Y. Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans. Int. J. Mol. Sci. 2012, 13, 4021-4032.
Lee J-K, Gopal R, Seo CH, Cheong H, Park Y. Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans. International Journal of Molecular Sciences. 2012; 13(4):4021-4032.
Lee, Jong-Kook; Gopal, Ramamourthy; Seo, Chang Ho; Cheong, Hyeonsook; Park, Yoonkyung. 2012. "Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans." Int. J. Mol. Sci. 13, no. 4: 4021-4032.