Int. J. Mol. Sci. 2012, 13(3), 2846-2862; doi:10.3390/ijms13032846
Article

Astragalin from Cassia alata Induces DNA Adducts in Vitro and Repairable DNA Damage in the Yeast Saccharomyces cerevisiae

Samuel Saito 1 email, Givaldo Silva 1 email, Regineide Xavier Santos 1 email, Grace Gosmann 2 email, Cristina Pungartnik 1,* email and Martin Brendel 1 email
1 Laboratório de Biologia de Fungos, Centro de Biotecnologia e Genética, Universidade Estadual de Santa Cruz, Ilhéus, BA, 45662-900, Brazil 2 Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90610-000, Brazil
* Author to whom correspondence should be addressed.
Received: 17 January 2012; in revised form: 22 February 2012 / Accepted: 22 February 2012 / Published: 5 March 2012
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Abstract: Reverse phase-solid phase extraction from Cassia alata leaves (CaRP) was used to obtain a refined extract. Higher than wild-type sensitivity to CaRP was exhibited by 16 haploid Saccharomyces cerevisiae mutants with defects in DNA repair and membrane transport. CaRP had a strong DPPH free radical scavenging activity with an IC50 value of 2.27 µg mL−1 and showed no pro-oxidant activity in yeast. CaRP compounds were separated by HPLC and the three major components were shown to bind to DNA in vitro. The major HPLC peak was identified as kampferol-3-O-β-D-glucoside (astragalin), which showed high affinity to DNA as seen by HPLC-UV measurement after using centrifugal ultrafiltration of astragalin-DNA mixtures. Astragalin-DNA interaction was further studied by spectroscopic methods and its interaction with DNA was evaluated using solid-state FTIR. These and computational (in silico) docking studies revealed that astragalin-DNA binding occurs through interaction with G-C base pairs, possibly by intercalation stabilized by H-bond formation.
Keywords: Cassia alata; astragalin; DNA binding; FTIR; Saccharomyces cerevisiae; antioxidant

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MDPI and ACS Style

Saito, S.; Silva, G.; Santos, R.X.; Gosmann, G.; Pungartnik, C.; Brendel, M. Astragalin from Cassia alata Induces DNA Adducts in Vitro and Repairable DNA Damage in the Yeast Saccharomyces cerevisiae. Int. J. Mol. Sci. 2012, 13, 2846-2862.

AMA Style

Saito S, Silva G, Santos RX, Gosmann G, Pungartnik C, Brendel M. Astragalin from Cassia alata Induces DNA Adducts in Vitro and Repairable DNA Damage in the Yeast Saccharomyces cerevisiae. International Journal of Molecular Sciences. 2012; 13(3):2846-2862.

Chicago/Turabian Style

Saito, Samuel; Silva, Givaldo; Santos, Regineide Xavier; Gosmann, Grace; Pungartnik, Cristina; Brendel, Martin. 2012. "Astragalin from Cassia alata Induces DNA Adducts in Vitro and Repairable DNA Damage in the Yeast Saccharomyces cerevisiae." Int. J. Mol. Sci. 13, no. 3: 2846-2862.

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