Int. J. Mol. Sci. 2012, 13(3), 2717-2726; doi:10.3390/ijms13032717
Article

Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect

1email, 1email, 1email, 1email, 1email, 1email, 2,* email and 1,* email
Received: 24 November 2011; in revised form: 4 February 2012 / Accepted: 14 February 2012 / Published: 29 February 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: (R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western blot showed that (R,R)ZX-5 up-regulated the expression of Akt, p-Akt (Thr473), eNOS and p-eNOS (Ser1177), down-regulated the expression of Cyclin D1 in human retinal endothelial cells and escalated the intracellular free Ca2+ concentration. Additionally, (R,R)ZX-5 inhibited the growth of blood vessels in the chick chorioallantoic membrane model. It is concluded that (R,R)ZX-5 promotes choroidal blood flow through PI3K/Akt-eNOS and Akt-Ca2+-eNOS pathways. Additionally, (R,R)ZX-5 can inhibit angiogenesis.
Keywords: (R,R)ZX-5; age-related macular degeneration; choroidal neovascularization; signal pathways; angiogenesis
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MDPI and ACS Style

Pan, L.; Hu, J.-L.; Wang, W.-J.; Zhang, X.-J.; Wei, J.; Liu, Z.-D.; Zhang, Y.-H.; Xu, H.-M. Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect. Int. J. Mol. Sci. 2012, 13, 2717-2726.

AMA Style

Pan L, Hu J-L, Wang W-J, Zhang X-J, Wei J, Liu Z-D, Zhang Y-H, Xu H-M. Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect. International Journal of Molecular Sciences. 2012; 13(3):2717-2726.

Chicago/Turabian Style

Pan, Li; Hu, Jia-Liang; Wang, Wen-Jing; Zhang, Xiao-Juan; Wei, Jin; Liu, Zhen-Dong; Zhang, Yi-Hua; Xu, Han-Mei. 2012. "Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect." Int. J. Mol. Sci. 13, no. 3: 2717-2726.

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