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Flavonoid Biosynthesis Genes Putatively Identified in the Aromatic Plant Polygonum minus via Expressed Sequences Tag (EST) Analysis
Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 Bangi Selangor, Malaysia
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia,43600 Bangi Selangor, Malaysia
* Author to whom correspondence should be addressed.
Received: 11 January 2012; in revised form: 31 January 2012 / Accepted: 2 February 2012 / Published: 28 February 2012
Abstract: P. minus is an aromatic plant, the leaf of which is widely used as a food additive and in the perfume industry. The leaf also accumulates secondary metabolites that act as active ingredients such as flavonoid. Due to limited genomic and transcriptomic data, the biosynthetic pathway of flavonoids is currently unclear. Identification of candidate genes involved in the flavonoid biosynthetic pathway will significantly contribute to understanding the biosynthesis of active compounds. We have constructed a standard cDNA library from P. minus leaves, and two normalized full-length enriched cDNA libraries were constructed from stem and root organs in order to create a gene resource for the biosynthesis of secondary metabolites, especially flavonoid biosynthesis. Thus, large‑scale sequencing of P. minus cDNA libraries identified 4196 expressed sequences tags (ESTs) which were deposited in dbEST in the National Center of Biotechnology Information (NCBI). From the three constructed cDNA libraries, 11 ESTs encoding seven genes were mapped to the flavonoid biosynthetic pathway. Finally, three flavonoid biosynthetic pathway-related ESTs chalcone synthase, CHS (JG745304), flavonol synthase, FLS (JG705819) and leucoanthocyanidin dioxygenase, LDOX (JG745247) were selected for further examination by quantitative RT-PCR (qRT-PCR) in different P. minus organs. Expression was detected in leaf, stem and root. Gene expression studies have been initiated in order to better understand the underlying physiological processes.
Keywords: cDNA library; expressed sequence tags; flavonoid biosynthesis; Polygonum minus; quantitative real-time PCR
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Roslan, N.D.; Yusop, J.M.; Baharum, S.N.; Othman, R.; Mohamed-Hussein, Z.-A.; Ismail, I.; Noor, N.M.; Zainal, Z. Flavonoid Biosynthesis Genes Putatively Identified in the Aromatic Plant Polygonum minus via Expressed Sequences Tag (EST) Analysis. Int. J. Mol. Sci. 2012, 13, 2692-2706.
Roslan ND, Yusop JM, Baharum SN, Othman R, Mohamed-Hussein Z-A, Ismail I, Noor NM, Zainal Z. Flavonoid Biosynthesis Genes Putatively Identified in the Aromatic Plant Polygonum minus via Expressed Sequences Tag (EST) Analysis. International Journal of Molecular Sciences. 2012; 13(3):2692-2706.
Roslan, Nur Diyana; Yusop, Jastina Mat; Baharum, Syarul Nataqain; Othman, Roohaida; Mohamed-Hussein, Zeti-Azura; Ismail, Ismanizan; Noor, Normah Mohd; Zainal, Zamri. 2012. "Flavonoid Biosynthesis Genes Putatively Identified in the Aromatic Plant Polygonum minus via Expressed Sequences Tag (EST) Analysis." Int. J. Mol. Sci. 13, no. 3: 2692-2706.