The Effect of C-Terminal Helix on the Stability of FF Domain Studied by Molecular Dynamics Simulation
AbstractTo investigate the effect of C-terminal helix on the stability of the FF domain, we studied the native domain FF3-71 from human HYPA/FBP11 and the truncated version FF3-60 with C-terminal helix being deleted by molecular dynamics simulations with GROMACS package and GROMOS 43A1 force field. The results indicated that the structures of truncated version FF3-60 were evident different from those of native partner FF3-71. Compared with FF3-71, the FF3-60 lost some native contacts and exhibited some similar structural characters to those of intermediate state. The C-terminal helix played a major role in stabilizing the FF3-71 domain. To a certain degree, the FF domain had a tendency to form an intermediate state without the C-terminal helix. In our knowledge, this was the first study to examine the role of C-terminal helix of FF domain in detail by molecular dynamics simulations, which was useful to understand the three-state folding mechanism of the small FF domain.
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Zhao, L.; Cao, Z.; Wang, J. The Effect of C-Terminal Helix on the Stability of FF Domain Studied by Molecular Dynamics Simulation. Int. J. Mol. Sci. 2012, 13, 1720-1732.
Zhao L, Cao Z, Wang J. The Effect of C-Terminal Helix on the Stability of FF Domain Studied by Molecular Dynamics Simulation. International Journal of Molecular Sciences. 2012; 13(2):1720-1732.Chicago/Turabian Style
Zhao, Liling; Cao, Zanxia; Wang, Jihua. 2012. "The Effect of C-Terminal Helix on the Stability of FF Domain Studied by Molecular Dynamics Simulation." Int. J. Mol. Sci. 13, no. 2: 1720-1732.