Next Article in Journal
Molecular Diagnostic and Pathogenesis of Hereditary Hemochromatosis
Previous Article in Journal
Nanoscopic and Photonic Ultrastructural Characterization of Two Distinct Insulin Amyloid States
Int. J. Mol. Sci. 2012, 13(2), 1481-1496; doi:10.3390/ijms13021481
Article

Pharmacological Mechanisms Underlying Gastroprotective Activities of the Fractions Obtained from Polygonum minus in Sprague Dawley Rats

1
,
2
,
3
,
4
 and
1,*
Received: 6 December 2011 / Revised: 11 January 2012 / Accepted: 18 January 2012 / Published: 1 February 2012
View Full-Text   |   Download PDF [394 KB, 19 June 2014; original version 19 June 2014]   |   Browse Figures

Abstract

The leaves of Polygonum minus were fractionated using an eluting solvent to evaluate the pharmacological mechanisms underlying the anti-ulcerogenic activity of P. minus. Different P. minus fractions were obtained and evaluated for their ulcer preventing capabilities using the ethanol induction method. In this study, Sprague Dawley rats weighing 150–200 g were used. Different parameters were estimated to identify the active fraction underlying the mechanism of the gastroprotective action of P. minus: the gastric mucus barrier, as well as superoxide dismutase, total hexosamine, and prostaglandin synthesis. Amongst the five fractions from the ethanolic extract of P. minus, the ethyl acetate:methanol 1:1 v/v fraction (F2) significantly (p < 0.005) exhibited better inhibition of ulcer lesions in a dose-dependent manner. In addition, rats pre-treated with F2 showed a significant elevation in superoxide dismutase (SOD), hexosamine and PGE2 levels in the stomach wall mucosa in a dose-dependent matter. Based on these results, the ethyl acetate:methanol 1:1 v/v fraction was considered to be the best fraction for mucous protection in the ethanol induction model. The mechanisms underlying this protection were attributed to the synthesis of antioxidants and PGE2.
Keywords: Polygonum minus; HPLC; UPLC-ESI-MS/MS; gastroprotective mechanisms Polygonum minus; HPLC; UPLC-ESI-MS/MS; gastroprotective mechanisms
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Qader, S.W.; Abdulla, M.A.; Chua, L.S.; Sirat, H.M.; Hamdan, S. Pharmacological Mechanisms Underlying Gastroprotective Activities of the Fractions Obtained from Polygonum minus in Sprague Dawley Rats. Int. J. Mol. Sci. 2012, 13, 1481-1496.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert