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Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology
Department of Bioengineering, Tatung University, No 40, Sec. 3, Zhongshan N. Rd., Taipei 104, Taiwan
* Author to whom correspondence should be addressed.
Received: 31 August 2012; in revised form: 23 October 2012 / Accepted: 8 November 2012 / Published: 13 November 2012
Abstract: Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.
Keywords: lipase; Burkholderia sp.; optimization; response surface methodology
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Lo, C.-F.; Yu, C.-Y.; Kuan, I.-C.; Lee, S.-L. Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 14889-14897.
Lo C-F, Yu C-Y, Kuan I-C, Lee S-L. Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology. International Journal of Molecular Sciences. 2012; 13(11):14889-14897.
Lo, Chia-Feng; Yu, Chi-Yang; Kuan, I-Ching; Lee, Shiow-Ling. 2012. "Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology." Int. J. Mol. Sci. 13, no. 11: 14889-14897.