Int. J. Mol. Sci. 2012, 13(11), 14889-14897; doi:10.3390/ijms131114889
Article

Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

Received: 31 August 2012; in revised form: 23 October 2012 / Accepted: 8 November 2012 / Published: 13 November 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.
Keywords: lipase; Burkholderia sp.; optimization; response surface methodology
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MDPI and ACS Style

Lo, C.-F.; Yu, C.-Y.; Kuan, I.-C.; Lee, S.-L. Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 14889-14897.

AMA Style

Lo C-F, Yu C-Y, Kuan I-C, Lee S-L. Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology. International Journal of Molecular Sciences. 2012; 13(11):14889-14897.

Chicago/Turabian Style

Lo, Chia-Feng; Yu, Chi-Yang; Kuan, I-Ching; Lee, Shiow-Ling. 2012. "Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology." Int. J. Mol. Sci. 13, no. 11: 14889-14897.

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