Int. J. Mol. Sci. 2012, 13(1), 534-539; doi:10.3390/ijms13010534
Short Note

A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China

1 Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China 2 Yunnan Reascend Tobacco Technology (Group) Co., Ltd., Kunming 650106, China 3 Faculty of Traditional Chinese Pharmacy, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China
* Author to whom correspondence should be addressed.
Received: 31 October 2011; in revised form: 23 December 2011 / Accepted: 28 December 2011 / Published: 4 January 2012
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Abstract: The genus Luculia Sweet contains about five species of small trees or shrubs and is a member of the family Rubiaceae (tribe Cinchoneae). Luculia yunnanensis is an endangered ornamental shrub endemic to southwest China. Only two natural populations of L. yunnanensis exist in the wild according to our field investigation. It can be inferred that L. yunnanensis is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-sterptavidin capture method, 24 primer sets were identified in two wild populations. Of these primers, 11 displayed polymorphisms and 13 were monomorphic. The number of alleles per locus ranged from two to four, values for observed and expected heterozygosities ranged from 0.000 to 0.833 and from 0.431 to 0.771, with averages of 0.389 and 0.614, respectively. These markers will be useful for further investigation of conservation of resources, selecting parental types in cross-breeding, evolution of this species at the molecular level and related research in Luculia species.
Keywords: genetic diversity; Luculia yunnanensis; microsatellite marker; polymorphism; population structure; Rubiaceae

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MDPI and ACS Style

Ma, H.; Wang, L.; Wan, Y.; Li, H.; Li, Z.; Liu, X.; Liang, N.; Li, W. A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China. Int. J. Mol. Sci. 2012, 13, 534-539.

AMA Style

Ma H, Wang L, Wan Y, Li H, Li Z, Liu X, Liang N, Li W. A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China. International Journal of Molecular Sciences. 2012; 13(1):534-539.

Chicago/Turabian Style

Ma, Hong; Wang, Lan; Wan, Youming; Li, Hongzhe; Li, Zhenghong; Liu, Xiuxian; Liang, Ning; Li, Wenjuan. 2012. "A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China." Int. J. Mol. Sci. 13, no. 1: 534-539.

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