Monitoring Insulin Aggregation via Capillary Electrophoresis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Detection of Insulin Oligomers Using CE with UV Detection
2.2. Effect of Salt Concentration on the Time Course for Insulin Oligomer Formation
2.3. Determination of Insulin Limit of Detection
2.4. Analysis of FITC Tracer Incorporation into Unlabeled Insulin
3. Materials and Methods
3.1. Materials
3.2. Insulin Preparation
3.3. Electrophoresis Conditions for UV and LIF Studies
3.4. Limit of Detection Studies
3.5. Oligomer Formation Assay
3.6. Statistical Analysis
4. Conclusion
Supplementary Material
ijms-12-09369-s001.pdfAcknowledgements
References
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NaCl concentration (mM) | Lag time for CE (h) 1 | Lag time for ThT Binding (h) 2 |
---|---|---|
100 | 6.7 ± 1.7 | 16 ± 1.0 *** |
150 | 5.0 ± 0.0 | 15 ± 1.4 *** |
250 | 7.6 ± 1.3 | 19 ± 2.4 *** |
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Pryor, E.; Kotarek, J.A.; Moss, M.A.; Hestekin, C.N. Monitoring Insulin Aggregation via Capillary Electrophoresis. Int. J. Mol. Sci. 2011, 12, 9369-9388. https://doi.org/10.3390/ijms12129369
Pryor E, Kotarek JA, Moss MA, Hestekin CN. Monitoring Insulin Aggregation via Capillary Electrophoresis. International Journal of Molecular Sciences. 2011; 12(12):9369-9388. https://doi.org/10.3390/ijms12129369
Chicago/Turabian StylePryor, Elizabeth, Joseph A. Kotarek, Melissa A. Moss, and Christa N. Hestekin. 2011. "Monitoring Insulin Aggregation via Capillary Electrophoresis" International Journal of Molecular Sciences 12, no. 12: 9369-9388. https://doi.org/10.3390/ijms12129369