A Sandwich Electrochemical Immunosensor Using Magnetic DNA Nanoprobes for Carcinoembryonic Antigen
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Different NP Complexes
2.2. Electrochemical Behaviors of the ECI
2.3. Optimization of Experimental Conditions
2.4. Detection of CEA
2.5. Precision, Reproducibility, and Stability
2.6. Application of the Immunosensor for Detecting CEA in Human Serum
3. Experimental Section
3.1. Reagents and Chemicals
3.2. Apparatus
3.3. Synthesis of Magnetic Cross-Liked Nanochain Probes
3.4. Designing of the Immunosensor Immunoassay Procedure for CEA Detection
3.5. Immunoassay Procedure for CEA Detection
4. Conclusions
Acknowledgments
References
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Immunosensor fabrication | Linear range (ng/mL) | DL (ng/mL) | ref. |
---|---|---|---|
HRP-anti-CEA/nano-Au/CS/CPCE | 0.5–25 | 0.22 | [19] |
anti-CEA/protein A/nano Au/ carbon fiber microelectrode (CFME) | 0.01–160 | 0.005 | [20] |
HRP–anti-CEA/nanogold/chitosan/SPCE | 2.5–40.0 | 1.1 | [21] |
anti-CEA/double nano-Au/poly(diallyldimethylammonium chloride /GCE | 0.5–120 | 0.2 | [22] |
HRP-anti-CEA/GA/CoFe2O4/CPE | 1.5–60 | 0.5 | [23] |
anti-CEA/Au/Fe3O4 NPs/SPCE | 0.01–150 | 0.005 | [24] |
anti-CEA/nano-Au/NiHCF/nano-Au/GCE | 0.5–160 | 0.1 | [25] |
This method | 0.008–200 | 0.005 | Our work |
CEA concentration (ng/mL) | |||||
---|---|---|---|---|---|
Sample | Detected by this method | Detected by ELISA | [CEA] Added in serum | Found after add CEA | Recovery (%) a |
1 | 2.1 | 2.0 | 2.0 | 4.3 | 105 |
2 | 4.2 | 4.0 | 4.0 | 8.0 | 95 |
3 | 10.2 | 10.5 | 10.0 | 21 | 107 |
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Gan, N.; Jia, L.; Zheng, L. A Sandwich Electrochemical Immunosensor Using Magnetic DNA Nanoprobes for Carcinoembryonic Antigen. Int. J. Mol. Sci. 2011, 12, 7410-7423. https://doi.org/10.3390/ijms12117410
Gan N, Jia L, Zheng L. A Sandwich Electrochemical Immunosensor Using Magnetic DNA Nanoprobes for Carcinoembryonic Antigen. International Journal of Molecular Sciences. 2011; 12(11):7410-7423. https://doi.org/10.3390/ijms12117410
Chicago/Turabian StyleGan, Ning, Liyong Jia, and Lei Zheng. 2011. "A Sandwich Electrochemical Immunosensor Using Magnetic DNA Nanoprobes for Carcinoembryonic Antigen" International Journal of Molecular Sciences 12, no. 11: 7410-7423. https://doi.org/10.3390/ijms12117410