A Three-Dimensional, Magnetic and Electroactive Nanoprobe for Amperometric Determination of Tumor Biomarkers
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Different Nanoparticle Complexes
2.2. Cyclic Voltammetry (CV) Measurements of the Different Nanoparticle Modified Electrodes
2.3. Optimization of Experimental Conditions
2.3.1. The Ratio of Fe3O4 Conjugated to MCNTs-hemin and Quantity of Fe3O4/hemin/MCNTs Nanocomposites on SPCE
2.3.2. The Maximum Quantity of Anti-AFP Immobilized on the Au NPs/Fe3O4/hemin/MCNTs Nanocomposite
2.3.3. Effect of Incubation Time and pH of the Working Buffer
2.4. Performance of the Immunosensor
2.5. Selectivity of the Immunosensor
2.6. Application of the Immunosensor to Serum Samples
3. Experimental Section
3.1. Chemicals and Reagents
3.2. Instruments and Measurements
3.3. Synthesis of Oleic Acid-Coated Fe3O4 NPs
3.4. Synthesis of Au NPs
3.5. Preparation of Anti-AFP and AFP Nanoprobes
3.6. The Detection Process of AFP on SPCE
4. Conclusions
Acknowledgements
References
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Added AFP Value (ng/mL) | 5 | 10 | 20 | 50 |
---|---|---|---|---|
ELISA (ng/mL) | 5.18 | 10.36 | 21.33 | 49.75 |
The Present Immunosensor (ng/mL) | 5.32 | 9.74 | 20.52 | 52.59 |
Relative Deviations (%) | 2.7 | −6.0 | −3.8 | 5.7 |
© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Meng, L.; Gan, N.; Li, T.; Cao, Y.; Hu, F.; Zheng, L. A Three-Dimensional, Magnetic and Electroactive Nanoprobe for Amperometric Determination of Tumor Biomarkers. Int. J. Mol. Sci. 2011, 12, 362-375. https://doi.org/10.3390/ijms12010362
Meng L, Gan N, Li T, Cao Y, Hu F, Zheng L. A Three-Dimensional, Magnetic and Electroactive Nanoprobe for Amperometric Determination of Tumor Biomarkers. International Journal of Molecular Sciences. 2011; 12(1):362-375. https://doi.org/10.3390/ijms12010362
Chicago/Turabian StyleMeng, Linghua, Ning Gan, Tianhua Li, Yuting Cao, Futao Hu, and Lei Zheng. 2011. "A Three-Dimensional, Magnetic and Electroactive Nanoprobe for Amperometric Determination of Tumor Biomarkers" International Journal of Molecular Sciences 12, no. 1: 362-375. https://doi.org/10.3390/ijms12010362