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Int. J. Mol. Sci. 2009, 10(9), 4009-4032; doi:10.3390/ijms10094009
Review

Experimental and Computational Characterization of Biological Liquid Crystals: A Review of Single-Molecule Bioassays

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Received: 25 August 2009; in revised form: 1 September 2009 / Accepted: 7 September 2009 / Published: 10 September 2009
(This article belongs to the Special Issue Liquid Crystals)
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Abstract: Quantitative understanding of the mechanical behavior of biological liquid crystals such as proteins is essential for gaining insight into their biological functions, since some proteins perform notable mechanical functions. Recently, single-molecule experiments have allowed not only the quantitative characterization of the mechanical behavior of proteins such as protein unfolding mechanics, but also the exploration of the free energy landscape for protein folding. In this work, we have reviewed the current state-of-art in single-molecule bioassays that enable quantitative studies on protein unfolding mechanics and/or various molecular interactions. Specifically, single-molecule pulling experiments based on atomic force microscopy (AFM) have been overviewed. In addition, the computational simulations on single-molecule pulling experiments have been reviewed. We have also reviewed the AFM cantilever-based bioassay that provides insight into various molecular interactions. Our review highlights the AFM-based single-molecule bioassay for quantitative characterization of biological liquid crystals such as proteins.
Keywords: single-molecule experiments; atomic force microscope (AFM); coarse-grained molecular dynamics simulation; coarse-grained model; in vitro molecular recognitions single-molecule experiments; atomic force microscope (AFM); coarse-grained molecular dynamics simulation; coarse-grained model; in vitro molecular recognitions
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Eom, K.; Yang, J.; Park, J.; Yoon, G.; Sohn, Y.S.; Park, S.; Yoon, D.S.; Na, S.; Kwon, T. Experimental and Computational Characterization of Biological Liquid Crystals: A Review of Single-Molecule Bioassays. Int. J. Mol. Sci. 2009, 10, 4009-4032.

AMA Style

Eom K, Yang J, Park J, Yoon G, Sohn YS, Park S, Yoon DS, Na S, Kwon T. Experimental and Computational Characterization of Biological Liquid Crystals: A Review of Single-Molecule Bioassays. International Journal of Molecular Sciences. 2009; 10(9):4009-4032.

Chicago/Turabian Style

Eom, Kilho; Yang, Jaemoon; Park, Jinsung; Yoon, Gwonchan; Sohn, Young S.; Park, Shinsuk; Yoon, Dae S.; Na, Sungsoo; Kwon, Taeyun. 2009. "Experimental and Computational Characterization of Biological Liquid Crystals: A Review of Single-Molecule Bioassays." Int. J. Mol. Sci. 10, no. 9: 4009-4032.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert