Int. J. Mol. Sci. 2009, 10(8), 3583-3598; doi:10.3390/ijms10083583
Article

Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

1,* email, 2email, 3email, 3email, 3email and 1email
Received: 2 June 2009; in revised form: 7 August 2009 / Accepted: 11 August 2009 / Published: 13 August 2009
(This article belongs to the Special Issue Biotic and Abiotic Stress)
View Full-Text   |   Download PDF [379 KB, uploaded 19 June 2014]
Abstract: The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVYO. The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVYNTN strain than in the case of strain PVYO. The relevance of NADP-malic enzyme in plants under stress conditions was discussed.
Keywords: NADP-malic enzyme; Nicotiana tabacum L.; Potato virus Y strain NTN (PVYNTN); Potato virus Y strain O (PVYO); biotic stress; real time-PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Doubnerová, V.; Müller, K.; Čeřovská, N.; Synková, H.; Spoustová, P.; Ryšlavá, H. Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity. Int. J. Mol. Sci. 2009, 10, 3583-3598.

AMA Style

Doubnerová V, Müller K, Čeřovská N, Synková H, Spoustová P, Ryšlavá H. Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity. International Journal of Molecular Sciences. 2009; 10(8):3583-3598.

Chicago/Turabian Style

Doubnerová, Veronika; Müller, Karel; Čeřovská, Noemi; Synková, Helena; Spoustová, Petra; Ryšlavá, Helena. 2009. "Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity." Int. J. Mol. Sci. 10, no. 8: 3583-3598.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert