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Int. J. Mol. Sci. 2009, 10(8), 3583-3598; doi:10.3390/ijms10083583
Article

Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

1,* , 2
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3
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3
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3
 and
1
1 Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic 2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, Prague 6, 165 02, Czech Republic 3 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, Prague 6, 160 00, Czech Republic
* Author to whom correspondence should be addressed.
Received: 2 June 2009 / Revised: 7 August 2009 / Accepted: 11 August 2009 / Published: 13 August 2009
(This article belongs to the Special Issue Biotic and Abiotic Stress)
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Abstract

The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVYO. The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVYNTN strain than in the case of strain PVYO. The relevance of NADP-malic enzyme in plants under stress conditions was discussed.
Keywords: NADP-malic enzyme; Nicotiana tabacum L.; Potato virus Y strain NTN (PVYNTN); Potato virus Y strain O (PVYO); biotic stress; real time-PCR NADP-malic enzyme; Nicotiana tabacum L.; Potato virus Y strain NTN (PVYNTN); Potato virus Y strain O (PVYO); biotic stress; real time-PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Doubnerová, V.; Müller, K.; Čeřovská, N.; Synková, H.; Spoustová, P.; Ryšlavá, H. Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity. Int. J. Mol. Sci. 2009, 10, 3583-3598.

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