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Int. J. Mol. Sci. 2009, 10(5), 2348-2366; doi:10.3390/ijms10052348
Article

Microscopic Investigation of Reversible Nanoscale Surface Size Dependent Protein Conjugation

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Received: 3 April 2009 / Revised: 14 May 2009 / Accepted: 18 May 2009 / Published: 20 May 2009
(This article belongs to the Special Issue Bioactive Nanoparticles (special issue))
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Abstract

1-40 coated 20 nm gold colloidal nanoparticles exhibit a reversible color change as pH is externally altered between pH 4 and 10. This reversible process may contain important information on the initial reversible step reported for the fibrillogenesis of Aβ (a hallmark of Alzheimer’s disease). We examined this reversible color change by microscopic investigations. AFM images on graphite surfaces revealed the morphology of Aβ aggregates with gold colloids. TEM images clearly demonstrate the correspondence between spectroscopic features and conformational changes of the gold colloid.
Keywords: amyloid beta; fibrillogenesis; Alzheimer’s disease; ovalbumin; gold nanoparticles; AFM; TEM amyloid beta; fibrillogenesis; Alzheimer’s disease; ovalbumin; gold nanoparticles; AFM; TEM
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yokoyama, K.; Cho, H.; Cullen, S.P.; Kowalik, M.; Briglio, N.M.; Hoops, H.J.; Zhao, Z.; Carpenter, M.A. Microscopic Investigation of Reversible Nanoscale Surface Size Dependent Protein Conjugation. Int. J. Mol. Sci. 2009, 10, 2348-2366.

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