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Int. J. Mol. Sci. 2009, 10(4), 1683-1696; doi:10.3390/ijms10041683
Communication

Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide

1, 2,* , 1, 2
 and 1
Received: 9 February 2009; in revised form: 3 April 2009 / Accepted: 10 April 2009 / Published: 17 April 2009
(This article belongs to the Special Issue Molecular Self-Assembly)
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Abstract: We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO2 surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO2 substrate, as demonstrated by having maximum 30-40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB.
Keywords: Lipid Vesicle; Supported Bilayer; Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Adsorption; Asymmetric Labeling Lipid Vesicle; Supported Bilayer; Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Adsorption; Asymmetric Labeling
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Reimhult, E.; Kasemo, B.; Höök, F. Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide. Int. J. Mol. Sci. 2009, 10, 1683-1696.

AMA Style

Reimhult E, Kasemo B, Höök F. Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide. International Journal of Molecular Sciences. 2009; 10(4):1683-1696.

Chicago/Turabian Style

Reimhult, Erik; Kasemo, Bengt; Höök, Fredrik. 2009. "Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide." Int. J. Mol. Sci. 10, no. 4: 1683-1696.



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