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Int. J. Mol. Sci. 2009, 10(4), 1683-1696; doi:10.3390/ijms10041683

Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide

1
Department of Applied Physics, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden
2
Laboratory for Surface Science and Technology, Department of Materials, Swiss Federal Institute of Technology Zurich (ETH Zurich), CH-8093 Zurich, Switzerland
*
Author to whom correspondence should be addressed.
Received: 9 February 2009 / Revised: 3 April 2009 / Accepted: 10 April 2009 / Published: 17 April 2009
(This article belongs to the Special Issue Molecular Self-Assembly)
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Abstract

We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO2 surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO2 substrate, as demonstrated by having maximum 30-40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB. View Full-Text
Keywords: Lipid Vesicle; Supported Bilayer; Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Adsorption; Asymmetric Labeling Lipid Vesicle; Supported Bilayer; Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Adsorption; Asymmetric Labeling
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Reimhult, E.; Kasemo, B.; Höök, F. Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide. Int. J. Mol. Sci. 2009, 10, 1683-1696.

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