Next Article in Journal
Chromatographic and Spectroscopic Identification and Recognition of Natural Dyes, Uncommon Dyestuff Components, and Mordants: Case Study of a 16th Century Carpet with Chintamani Motifs
Previous Article in Journal
Interplay between P-Glycoprotein Expression and Resistance to Endoplasmic Reticulum Stressors
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(2), 338; https://doi.org/10.3390/molecules23020338

A Highly Sensitive Immunochromatographic Strip Test for Rapid and Quantitative Detection of Saikosaponin d

1
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
2
Jiangsu Kanion Pharmaceutical Co., Ltd., 58 Jiangning Industrial Park Kangyuan Road, Lianyungang, Jiangsu, 210000, China
3
School of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, China
4
Centre of Scientific Experiment, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing 100029, China
*
Authors to whom correspondence should be addressed.
Received: 11 December 2017 / Revised: 23 January 2018 / Accepted: 1 February 2018 / Published: 6 February 2018
View Full-Text   |   Download PDF [1662 KB, uploaded 9 February 2018]   |  

Abstract

A quantitative lateral-flow immunoassay using gold nanoparticles (AuNPs) conjugated with a monoclonal antibody (MAb) against saikosaponin d (SSd) was developed for the analysis of SSd. The AuNPs were prepared in our laboratory. The AuNPs were polyhedral, with an average diameter of approximately 18 nm. We used the conjugation between AuNPs and MAbs against SSd to prepare immunochromatographic strips (ICSs). For the quantitative experiment, the strips with the test results were scanned using a membrane strip reader, and a detection curve (regression equation, y = −0.113ln(x) + 1.5451, R2 = 0.983), representing the averages of the scanned data, was obtained. This curve was linear from 96 ng/mL to 150 μg/mL, and the IC50 value was 10.39 μg/mL. In this study, we bring the concept of POCT (point-of-care testing) to the measurement of TCM compounds, and this is the first report of quantitative detection of SSd by an ICS. View Full-Text
Keywords: colloidal gold; immunochromatographic strip; monoclonal antibody; saikosaponin d; rapid detection colloidal gold; immunochromatographic strip; monoclonal antibody; saikosaponin d; rapid detection
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Zhang, Y.; Xiao, W.; Kong, H.; Cheng, J.; Yan, X.; Zhang, M.; Wang, Q.; Qu, H.; Zhao, Y. A Highly Sensitive Immunochromatographic Strip Test for Rapid and Quantitative Detection of Saikosaponin d. Molecules 2018, 23, 338.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top