Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules
AbstractIn the present report, we are making the proof of concept of cell small populations (from 1 to 100 cells) spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume (30 nL), directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging (SPRi) experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen (PSA) and β-2-microglobulin (B2M) secreted by prostate cancer cells following induction by dihydrotestosterone (DHT). Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip. View Full-Text
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Berthuy, O.I.; Blum, L.J.; Marquette, C.A. Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules. Biosensors 2016, 6, 2.
Berthuy OI, Blum LJ, Marquette CA. Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules. Biosensors. 2016; 6(1):2.Chicago/Turabian Style
Berthuy, Ophélie I.; Blum, Loïc J.; Marquette, Christophe A. 2016. "Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules." Biosensors 6, no. 1: 2.