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Antioxidants 2015, 4(2), 359-372; doi:10.3390/antiox4020359

Discrimination between Leave of Apocynum venetum and Its Adulterant, A. pictum Based on Antioxidant Assay and Chemical Profiles Combined with Multivariate Statistical Analysis

1
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Shenzhen 518057, China
2
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China
3
The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Antonio Segura-Carretero and David Arráez-Román
Received: 31 March 2015 / Revised: 23 April 2015 / Accepted: 24 April 2015 / Published: 8 May 2015
(This article belongs to the Special Issue Analytical Determination of Polyphenols)
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Abstract

An integrated approach including chemical and biological assessments was developed to investigate the differences between Apocynum venetum L. (AV) and its adulterant, Apocynum pictum Schrenk (AP). Ten flavonoids were tentatively identified by ultra-visible and mass spectra data. The chemical component, hyperoside, was identified as a critical parameter for discrimination of two species from the results of principal component analysis (PCA) and quantitative analysis. The anti-oxidative power of the herbal extracts were determined using 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay and H2O2-induced cell damage on LO2 cells. The results of the biological assays suggested that the chemical differences between AV and AP do lead to difference in activity and AV is demonstrated to have higher anti-oxidant activity. View Full-Text
Keywords: Apocynum venetum L.; Apocynum pictum Schrenk; HPLC fingerprints; free radical scavenging capacity Apocynum venetum L.; Apocynum pictum Schrenk; HPLC fingerprints; free radical scavenging capacity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chan, C.-O.; Lau, C.-C.; Ng, Y.-F.; Xu, L.-J.; Chen, S.-B.; Chan, S.-W.; Mok, D.K.-W. Discrimination between Leave of Apocynum venetum and Its Adulterant, A. pictum Based on Antioxidant Assay and Chemical Profiles Combined with Multivariate Statistical Analysis. Antioxidants 2015, 4, 359-372.

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